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Title: Gene Expression in B-1 Cells from Lupus-Prone Mice
Authors: Novaes e Brito, Ronni Romulo [UNIFESP]
Xander, Patricia [UNIFESP]
Perez, Elizabeth C. [UNIFESP]
Maricato, Juliana Terzi [UNIFESP]
Laurindo, Maria Fl. [UNIFESP]
De Lorenzo, Beatriz H. P. [UNIFESP]
Pellegrino, Renata [UNIFESP]
Bernardo, Viviane [UNIFESP]
Lopes, Jose Daniel [UNIFESP]
Mariano, Mario [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Ctr Univ Sao Camilo
Keywords: Autoimmunity
B-1 cells
Issue Date: 1-Jan-2014
Publisher: Informa Healthcare
Citation: Immunological Investigations. London: Informa Healthcare, v. 43, n. 7, p. 675-692, 2014.
Abstract: New Zealand Black X New Zealand White F1 [(NZB/NZW) F1] mice develop an autoimmune condition with similarities to human systemic lupus erythematosus (SLE). in this study, we demonstrate that B-1 cells, which have previously been reported to be involved in several autoimmune diseases, have altered gene expression in these mice. RNA was extracted from purified B-1 cells of disease-free C57BL/6 mice and lupus-prone (NZB/NZW) F1 mice. Gene expression was analysed using DNA microarray techniques and validated by real time reverse transcriptase polymerase chain reaction (RT-PCR). in (NZB/NZW) F1 mice, some genes had altered expression patterns compared to disease-free controls. Specifically, the upregulation of Ifitm1, Pvrl2 and Ifi202b and downregulation of Trp53bp1 mRNA were observed in (NZB/NZW) F1 mice. These genes are known to be associated with autoimmune diseases. This pattern of gene expression in B-1 cells could understanding of the pathogenesis of SLE. Thus, it is reasonable to hypothesise that the altered gene expression observed in B-1 cells in our experimental model is important for SLE prognosis and therapy, and these implications are discussed herein.
ISSN: 0882-0139
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