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Title: Brown spider (Loxosceles intermedia) venom triggers endothelial cells death by anoikis
Authors: Nowatzki, Jenifer
Sene, Reginaldo Vieira
Paludo, Katia Sabrina
Rizzo, Luiz Eduardo
Souza-Fonseca-Guimaraes, Fernando
Veiga, Silvio Sanches
Nader, Helena Bonciani [UNIFESP]
Franco, Celia Regina C.
Trindade, Edvaldo S.
Univ Fed Parana
Universidade Federal de São Paulo (UNIFESP)
Estadual Univ Parana
Keywords: Loxosceles intermedia
Endothelial cells
Issue Date: 1-Sep-2012
Publisher: Elsevier B.V.
Citation: Toxicon. Oxford: Pergamon-Elsevier B.V., v. 60, n. 3, p. 396-405, 2012.
Abstract: Brown spider (Loxosceles sp.) venom affects the endothelium of vessels and triggers disruptive activity in the subendothelial matrix. the vascular disorders observed after venom exposure include leukocyte and platelet activation, disseminated intravascular coagulation, an increase in vessel permeability and hemorrhage into the dermis. in this study, we report additional evidence regarding the mechanism of endothelial cell cytotoxicity induced by Loxosceles intermedia venom. Exposure to venom led to endothelial cell detachment in a time-dependent manner. Loss of cell anchorage and cell-cell adhesion following venom exposure was accompanied by changes in the distribution of the alpha(5)beta(1) integrin and VE-cadherin. An ultrastructural analysis of cells treated with venom revealed morphological alterations characteristic of apoptosis. Moreover, after venom exposure, the ratio between Bax and Bcl-2 proteins was disturbed in favor of Bax. in addition, late apoptosis was only observed in cells detached by the action of venom. Accordingly, there was no increase in apoptosis when cells were exposed to L. intermedia venom in suspension, suggesting that the loss of cell anchorage provides the signal to initiate apoptosis. Thus. L. intermedia venom likely triggers endothelial cell death indirectly through an apoptotic mechanism known as anoikis. (C) 2012 Elsevier B.V. All rights reserved.
ISSN: 0041-0101
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