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Title: Target of Rapamycin (TOR)-like 1 Kinase Is Involved in the Control of Polyphosphate Levels and Acidocalcisome Maintenance in Trypanosoma brucei
Authors: Jesus, Teresa Cristina Leandro de [UNIFESP]
Tonelli, Renata Rosito [UNIFESP]
Nardelli, Sheila Cristina [UNIFESP]
Augusto, Leonardo da Silva [UNIFESP]
Motta, Maria Cristina M.
Girard-Dias, Wendell
Miranda, Kildare
Ulrich, Paul
Jimenez, Veronica
Barquilla, Antonio
Navarro, Miguel
Docampo, Roberto
Schenkman, Sergio [UNIFESP]
Univ Georgia
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro (UFRJ)
Inst Nacl Metrol Normalizacao & Qualidade Ind
Consejo Super Invest Cient
Issue Date: 30-Jul-2010
Publisher: Amer Soc Biochemistry Molecular Biology Inc
Citation: Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 285, n. 31, p. 24131-24140, 2010.
Abstract: Target of rapamycin (TOR) kinases are highly conserved protein kinases that integrate signals from nutrients and growth factors to coordinate cell growth and cell cycle progression. It has been previously described that two TOR kinases control cell growth in the protozoan parasite Trypanosoma brucei, the causative agent of African trypanosomiasis. Here we studied an unusual TOR-like protein named TbTOR-like 1 containing a PDZ domain and found exclusively in kinetoplastids. TbTOR-like 1 localizes to unique cytosolic granules. After hyperosmotic stress, the localization of the protein shifts to the cell periphery, different from other organelle markers. Ablation of TbTOR-like 1 causes a progressive inhibition of cell proliferation, producing parasites accumulating in the S/G(2) phase of the cell cycle. TbTOR-like 1 knocked down cells have an increased area occupied by acidic vacuoles, known as acidocalcisomes, and are enriched in polyphosphate and pyrophosphate. These results suggest that TbTOR-like 1 might be involved in the control of acidocalcisome and polyphosphate metabolism in T. brucei.
ISSN: 0021-9258
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