Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/31903
Title: Vitamin C and E supplementation prevents mitochondrial damage of ileum myocytes caused by intense and exhaustive exercise training
Authors: Rosa, Eloi F.
Ribeiro, Rafael Ferreira
Pereira, Felipe M. T.
Freymueller, Edna [UNIFESP]
Aboulafia, Jeannine
Nouailhetas, Viviane L. A. [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Ctr Univ Sao Camilo
Keywords: C57BL/6 mice
intestine
mitochondria
Issue Date: 1-Nov-2009
Publisher: Amer Physiological Soc
Citation: Journal of Applied Physiology. Bethesda: Amer Physiological Soc, v. 107, n. 5, p. 1532-1538, 2009.
Abstract: Rosa EF, Ribeiro RF, Pereira FM, Freymuller E, Aboulafia J, Nouailhetas VL. Vitamin C and E supplementation prevents mitochondrial damage of ileum myocytes caused by intense and exhaustive exercise training. J Appl Physiol 107: 1532-1538, 2009. First published August 20, 2009; doi: 10.1152/japplphysiol.91166.2008.Intense and exhaustive exercise (IEE) is associated with oxidative stress in skeletal muscle, and we recently reported that intestine is sensitive to IEE. in the present study, we investigated the possible relationship between the effects of IEE on morphology and oxidative markers in the ileum and isolated mitochondria. C57BL/6 mice were ascribed either to a control group comprising two subgroups, one sedentary and another exercised for 10 days (E10), or to a corresponding supplemented control group again comprising two subgroups, one sedentary and another exercised for 10 days (E10-V). the IEE program consisted of a single daily treadmill running session at 85% of V(max), until animal exhaustion. Vitamins C (10 mg/kg) and E (10 mg/kg) were concurrently intraperitoneally administered 2 h before the exercise sessions. IEE was shown to cause 1) impairment of ileum internal membrane mitochondria verified by ultramicrography analysis; 2) increase in ileum carbonyl content (117%) and reduction in antioxidant capacity (36%); 3) increase in mitochondria carbonyl content (38%), increase in the percentage of ruptured mitochondria 25.3%), increase in superoxide dismutase activity (186%), and reduction in citrate synthase activity (40.4%) compared with control animals. Observations in the vitamin-supplemented exercised animals (E10-V) were 1) healthy appearance of myocyte mitochondria; 2) decrease in ileum carbonyl content (66%) and increase in antioxidant capacity (53%); 3) decrease in mitochondria carbonyl content (43%), decrease in the percentage of ruptured mitochondria (30%), slight increase in superoxide dismutase activity (7%), and significant increase in citrate synthase activity (121%) compared with E10 animals. Therefore, the present results strongly corroborate the hypothesis that IEE leads to marked disturbances in intestinal mitochondria, mainly in redox status, and affects whole intestinal redox status.
URI: http://repositorio.unifesp.br/handle/11600/31903
ISSN: 8750-7587
Other Identifiers: http://dx.doi.org/10.1152/japplphysiol.91166.2008
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