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Title: Glycosaminoglycan chains from alpha(5)beta(1) integrin are involved in fibronectin-dependent cell migration
Authors: Franco, Celia R. C. [UNIFESP]
Trindade, Edvaldo S. [UNIFESP]
Rocha, Hugo A. O. [UNIFESP]
Silveira, Rafael Bertoni da [UNIFESP]
Paludo, Katia Sabrina
Chammas, Roger
Veiga, Silvio S.
Nader, Helena B. [UNIFESP]
Dietrich, Carl P. [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Univ Fed Parana
Univ Fed Rio Grande do Norte
Universidade de São Paulo (USP)
Keywords: integrin
migration on fibronectin
adhesion on fibronectin
Issue Date: 1-Aug-2009
Publisher: Canadian Science Publishing, Nrc Research Press
Citation: Biochemistry and Cell Biology-biochimie Et Biologie Cellulaire. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 87, n. 4, p. 677-686, 2009.
Abstract: alpha(5)beta(1) integrin from both wild-type CHO cells (CHO-K1) and deficient in proteoglycan biosynthesis (CHO-745) is post-translationally modified by glycosaminoglycan chains. We demonstrated this using [S-35]sulfate metabolic labeling of the cells, enzymatic degradation, immunoprecipitation reaction with monoclonal antibody, fluorescence microscopy, and flow cytometry. the alpha(5)beta(1) integrin heterodimer is a hybrid proteoglycan containing both chondroitin and heparan sulfate chains. Xyloside inhibition of sulfate incorporation into alpha(5)beta(1) integrin also supports that integrin is a proteoglycan. Also. cells grown with xyloside adhered on fibronectin with no alteration in alpha(5)beta(1) integrin expression. However, haptotactic motility on fibronectin declined in cells grown with xyloside or chlorate as compared with controls. Thus, alpha(5)beta(1) integrin is a proteoglycan and the glycosaminoglycan chains of the integrin influence cell motility on fibronectin. Similar glycosylation of alpha(5)beta(1) integrin was observed in other normal and malignant cells, suggesting that this modification is conserved and important in the function of this integrin. Therefore, these glycosaminoglycan chains of alpha(5)beta(1) integrin are involved in cellular migration on fibronectin.
ISSN: 0829-8211
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