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dc.contributor.authorMufalo, Bruno C.
dc.contributor.authorGentil, Fernanda
dc.contributor.authorBargieri, Daniel Y. [UNIFESP]
dc.contributor.authorCosta, Fabio Trindade Maranhão [UNIFESP]
dc.contributor.authorRodrigues, Mauricio M. [UNIFESP]
dc.contributor.authorSoares, Irene S.
dc.identifier.citationMicrobes and Infection. Amsterdam: Elsevier B.V., v. 10, n. 12-13, p. 1266-1273, 2008.
dc.description.abstractThe Apical Membrane Antigen-1 (AMA-1) of Plasmodium sp. has been suggested as a vaccine candidate against malaria. This protein seems to be involved in merozoite invasion and its extra-cellular portion contains three distinct domains: DI, DII, and DIII. Previously, we described that Plasmodium vivax AMA-1 (PvAMA-1) ectodomain is highly immunogenic in natural human infections. Here, we expressed each domain, separately or in combination (DI-II or DII-III), as bacterial recombinant proteins to map immunodominant epitopes within the PvAMA-1 ectodomain. IgG recognition was assessed by ELISA using sera of P. vivax-infected individuals collected from endemic regions of Brazil or antibodies raised in immunized mice. the frequencies of responders to recombinant proteins containing the DII were higher than the others and similar to the ones observed against the PvAMA-1 ectodomain. Moreover, ELISA inhibition assays using the PvAMA-1 ectodomain as substrate revealed the presence of many common epitopes within DI-II that are recognized by human immune antibodies. Finally, immunization of mice with the PvAMA-1 ectodomain induced high levels of antibodies predominantly to DI-II. Together, our results indicate that DII is particularly immunogenic during natural human infections, thus indicating that this region could be used as part of an experimental sub-unit vaccine to prevent vivax malaria. (C) 2008 Elsevier Masson SAS. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.publisherElsevier B.V.
dc.relation.ispartofMicrobes and Infection
dc.rightsAcesso restrito
dc.subjectPlasmodium vivaxen
dc.subjectApical Membrane Antigen-1en
dc.titlePlasmodium vivax apical membrane antigen-1: comparative recognition of different domains by antibodies induced during natural human infectionen
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.description.affiliationUniv São Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, CINTERGEN, BR-04044010 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Dept Parasitol, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, CINTERGEN, BR-04044010 São Paulo, Brazil
dc.description.sponsorshipIDFAPESP: 2004/00768-7
dc.description.sponsorshipIDFAPERJ: E-26/110.305/2007
dc.description.sponsorshipIDCNPq: 420067/2005-1
dc.description.sourceWeb of Science
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