Please use this identifier to cite or link to this item:
Title: A Role for galectin-3 in renal tissue damage triggered by ischemia and reperfusion injury
Authors: Fernandes Bertocchi, Ana Paula [UNIFESP]
Campanhole, Gabriela [UNIFESP]
Mei Wang, Pamella Huey [UNIFESP]
Goncalves, Giselle Martins [UNIFESP]
Damiao, Marcio Jose [UNIFESP]
Cenedeze, Marcos Antonio [UNIFESP]
Beraldo, Felipe Caetano
Antunes Teixeira, Vicente de Paula
Reis, Marlene Antonia dos
Mazzali, Marilda
Pacheco-Silva, Alvaro [UNIFESP]
Saraiva Camara, Niels Olsen [UNIFESP]
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Keywords: galectin-3
IL-1 beta
ischemia and reperfusion injury
Issue Date: 1-Oct-2008
Publisher: Wiley-Blackwell
Citation: Transplant International. Malden: Wiley-Blackwell, v. 21, n. 10, p. 999-1007, 2008.
Abstract: Ischemic-reperfusion injury (IRI) triggers an inflammatory response involving neutrophils/macrophages, lymphocytes and endothelial cells. Galectin-3 is a multi-functional lectin with a broad range of action such as promotion of neutrophil adhesion, induction of oxidative stress, mastocyte migration and degranulation, and production of pro-inflammatory cytokines. the aim of this study was evaluate the role of galectin-3 in the inflammation triggered by IRI. Galectin-3 knockout (KO) and wild type (wt) mice were subjected to 45 min of renal pedicle occlusion. Blood and kidney samples were collected at 6, 24, 48 and 120 h. Blood urea was analyzed enzymatically, while MCP-1, IL-6 and IL-1 beta were studied by real-time PCR. Reactive oxygen species (ROS) was investigated by flow cytometry. Morphometric analyses were performed at 6, 24, 48 and 120 h after reperfusion. Urea peaked at 24 h, being significantly lower in knockout animals (wt = 264.4 +/- 85.21 mg/dl vs. gal-3 KO = 123.74 +/- 29.64 mg/dl, P = 0.001). Galectin-3 knockout animals presented less acute tubular necrosis and a more prominent tubular regeneration when compared with controls concurrently with lower expression of MCP-1, IL-6, IL-1 beta, less macrophage infiltration and lower ROS production at early time points. Galectin-3 seems to play a role in renal IRI involving the secretion of macrophage-related chemokine, pro-inflammatory cytokines and ROS production.
ISSN: 0934-0874
Other Identifiers:
Appears in Collections:Em verificação - Geral

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.