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dc.contributor.authorDi Marco, Giovana Seno
dc.contributor.authorColucci, Juliana Almada [UNIFESP]
dc.contributor.authorFernandes, Fernanda Barrinha [UNIFESP]
dc.contributor.authorVio, Carlos Pablo
dc.contributor.authorSchor, Nestor [UNIFESP]
dc.contributor.authorCasarini, Dulce Elena [UNIFESP]
dc.identifier.citationInternational Journal of Biochemistry & Cell Biology. Oxford: Pergamon-Elsevier B.V., v. 40, n. 4, p. 747-754, 2008.
dc.description.abstractDiabetes mellitus is a frequent cause of kidney function damage with diabetic nephropathy being predominantly related to glomerular dysfunction. Diabetes is capable of interfering with distinct hormonal systems, as well as catecholamine metabolism. Since mesangial cells, the major constituent of renal glomerulus, constitute a potential site for catecholamine production, the present study was carried out to investigate alterations in catecholamine metabolism in cultured mesangial cells from the nonobese diabetic mouse, a well-established model for type I diabetes. We evaluated mesangial cells from normoglycemic and hyperglycemic nonobese diabetic mice, as well as cells from normoglycemic Swiss tnice as control. Mesangial cells from normoglycemic mice presented similar profiles concerning all determinations. However, cells isolated from hyperglycemic animals presented increased dopamine and norepinephrine production/secretion. Among the studied mechanisms, we observed an upregulation of tyrosine hydroxylase expression accompanied by increased tetrahydrobiopterin consumption, the tyrosine hydroxylase enzymatic cofactor. However, this increase in synthetic pathways was followed by decreased monoamine oxidase activity, which corresponds to the major metabolic pathway of catecholamines in mesangial cells. in addition, Whole kidney homogenates from diabetic animals also presented increased dopamine and norepinephrine levels when compared to normoglycemic animals. Thus, our results suggest that diabetes alters catecholamine production by interfering with both synthesizing and degrading enzymes, suggesting a possible role of catecholamine in the pathogenesis of acute and chronic renal complications of diabetes mellitus. (C) 2007 Elsevier B.V. All rights reserved.en
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Journal of Biochemistry & Cell Biology
dc.rightsAcesso restrito
dc.subjectmesangial cellen
dc.subjectnonobese diabetic miceen
dc.titleDiabetes induces changes of catecholamines in primary mesangial cellsen
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Clin Muenster
dc.contributor.institutionPontificia Univ Catolica Chile
dc.description.affiliationUniversidade Federal de São Paulo, Div Nephrol, Dept Med, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniv Clin Muenster, D-48149 Munster, Germany
dc.description.affiliationPontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Physiol Sci, Santiago, Chile
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Nephrol, Dept Med, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
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