Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/27974
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dc.contributor.authorKerbauy, Fábio Rodrigues [UNIFESP]
dc.contributor.authorColleoni, Gisele Wally Braga [UNIFESP]
dc.contributor.authorSaad, Sara Teresinha Olalla
dc.contributor.authorSilva, MRR
dc.contributor.authorAlves, Antonio Correa [UNIFESP]
dc.contributor.authorAguiar, Kátia Cilene Carozzi [UNIFESP]
dc.contributor.authorAlbuquerque, Dulcineia M. [UNIFESP]
dc.contributor.authorKobarg, J.
dc.contributor.authorSeixas, Maria Teresa [UNIFESP]
dc.contributor.authorKerbauy, José [UNIFESP]
dc.date.accessioned2016-01-24T12:37:25Z-
dc.date.available2016-01-24T12:37:25Z-
dc.date.issued2004-10-01
dc.identifierhttp://dx.doi.org/10.1080/10428190410001713170
dc.identifier.citationLeukemia & Lymphoma. Abingdon: Taylor & Francis Ltd, v. 45, n. 10, p. 2071-2078, 2004.
dc.identifier.issn1042-8194
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27974-
dc.description.abstractDiffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin's lymphoma. Although the presence of p53 gene mutations has been considered as a bad prognostic feature in DLBCL, its clinical significance is still controversial. the aims of this study were: detect the presence of mutations in exons 5 to 9 of the p53 gene and correlate it to prognosis in DLBCL. Fifty-one DLBCL patients were enrolled in this study. Expression of p53 was evaluated by immunohistochemistry. the screening of p53 mutations was performed using PCR-SSCP methods. Cases showing a mobility shift on SSCP electrophoresis were analyzed by automatic sequencing. We could identify 8 missense mutations in 6 of 48 cases (12.5%). in addition, we found a known polymorphism at codon 213 and 2 instances of silent mutations. of all mutations/polymorphisms found, 7 (64%) were localized in codons previously described as p53 hot spots in NHL cases. of the remaining alterations (4 or 36%), 2 mutations were localized in codons previously described as hot spots for p53 in other tumors and 2 (codon 142 of the exon 5 and codon 195 of the exon 6), in codons not described as hot spots for p53 up to now. the presence of missense mutations in exons 5 to 9 of p53 gene had adverse impact on overall survival (P=0.020). Cox's Regression Model identified that high-risk International Prognostic Index (IPI) and p53 gene mutations have independent negative impact on OS. Therefore, the association of IPI with cellular factors, such as p53 mutation, can be very helpful in deciding when we should indicate more aggressive therapies in patients with DLBCL, to somehow increase the chance of cure in these patients.en
dc.format.extent2071-2078
dc.language.isoeng
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofLeukemia & Lymphoma
dc.rightsAcesso restrito
dc.subjectnon-Hodgkin's lymphomasen
dc.subjectP53 proteinen
dc.subjectp53 gene mutationen
dc.subjectprognosisen
dc.titleDetection and possible prognostic relevance of p53 gene mutations in diffuse large B-cell lymphoma. An analysis of 51 cases and review of the literatureen
dc.typeArtigo
dc.rights.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionCtr Biol Mol Estrutural
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP EPM, Discipline Hematol & Hemotherapy, São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Hemoctr UNICAMP, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP EPM, Dept Pathol, São Paulo, Brazil
dc.description.affiliationCtr Biol Mol Estrutural, Lab Nacl Luz Sincrotron, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP EPM, Discipline Hematol & Hemotherapy, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP EPM, Dept Pathol, São Paulo, Brazil
dc.identifier.doi10.1080/10428190410001713170
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000223523600014
Appears in Collections:Artigo
Artigo

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