Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stress

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dc.citation.issue4
dc.citation.volumev. 50
dc.contributor.authorAdami, L. N. G. [UNIFESP]
dc.contributor.authorBelardin, Larissa Berloffa [UNIFESP]
dc.contributor.authorLima, B. T. [UNIFESP]
dc.contributor.authorJeremias, J. T. [UNIFESP]
dc.contributor.authorAntoniassi, Mariana Pereira [UNIFESP]
dc.contributor.authorOkada, Fatima Kazue [UNIFESP]
dc.contributor.authorBertolla, Ricardo Pimenta [UNIFESP]
dc.coverageHoboken
dc.date.accessioned2020-07-20T16:30:56Z
dc.date.available2020-07-20T16:30:56Z
dc.date.issued2018
dc.description.abstractThe aim of this study was to evaluate the antioxidant effect of in vitro supplementation with vitamin E in human spermatozoon incubated with an oxidative stress inducer. In this study, semen samples from 30 patients were collected and with one aliquot we performed semen analysis according to WHO. The remaining volume was divided into four aliquots: group C: incubated with BWW mediumen
dc.description.abstractgroup I: incubated with 5mmol 1-1 hydrogen peroxideen
dc.description.abstractgroup A: incubated with 40mol 1-1 vitamin Een
dc.description.abstractand group AI: incubated with both them. After incubations, sperm functional analyses were performed and included: evaluation of oxidative stress, acrosome integrity, mitochondrial activity and DNA fragmentation. Groups were compared using a Friedman test with Bonferroni post hoc (=5%). In this study, we observed that in group I there was a decrease in acrosome integrity and mitochondrial activity, and an increase in DNA fragmentation, when compared to group C. Group AI showed an increase in acrosome integrity and mitochondrial activity when compared with group I. Based on our findings, we conclude that the vitamin E supplementation had a positive effect in protecting human spermatozoon from induced oxidative stress.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, Sao Paulo, Brazil
dc.description.affiliationHosp Sao Paulo, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, Sao Paulo, Brazil
dc.description.affiliationUnifespHosp Sao Paulo, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPESP
dc.description.sponsorshipCNPq
dc.description.sponsorshipIDFAPESP: 2015/02955-3
dc.description.sponsorshipIDCNPq: 306616/2013-0
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1111/and.12959
dc.identifier.citationAndrologia. Hoboken, v. 50, n. 4, p. -, 2018.
dc.identifier.doi10.1111/and.12959
dc.identifier.issn0303-4569
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55602
dc.identifier.wosWOS:000429536900018
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofAndrologia
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectantioxidanten
dc.subjectmale infertilityen
dc.subjectoxidative stressen
dc.subjectreactive oxygen speciesen
dc.subjectvitamin Een
dc.titleEffect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stressen
dc.typeinfo:eu-repo/semantics/article
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