Proteomic profiling of the rat hypothalamus

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dc.contributor.authorPedroso, Amanda Paula [UNIFESP]
dc.contributor.authorWatanabe, Regina Lucia Harumi [UNIFESP]
dc.contributor.authorAlbuquerque, Kelse Tibau de [UNIFESP]
dc.contributor.authorTelles, Monica Marques [UNIFESP]
dc.contributor.authorAndrade, Maria Claudina Camargo de [UNIFESP]
dc.contributor.authorPerez, Juliana Dineia [UNIFESP]
dc.contributor.authorSakata, Maisa Mayumi [UNIFESP]
dc.contributor.authorDe Lima-Stein, Mariana Leão [UNIFESP]
dc.contributor.authorEstadella, Debora [UNIFESP]
dc.contributor.authorNascimento, Claudia Maria da Penha Oller do [UNIFESP]
dc.contributor.authorOyama, Lila Missae [UNIFESP]
dc.contributor.authorRosa, Jose Cesar
dc.contributor.authorCasarini, Dulce Elena [UNIFESP]
dc.contributor.authorRibeiro, Eliane Beraldi [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:27:07Z
dc.date.available2016-01-24T14:27:07Z
dc.date.issued2012-04-20
dc.description.abstractBackground: the hypothalamus plays a pivotal role in numerous mechanisms highly relevant to the maintenance of body homeostasis, such as the control of food intake and energy expenditure. Impairment of these mechanisms has been associated with the metabolic disturbances involved in the pathogenesis of obesity. Since rodent species constitute important models for metabolism studies and the rat hypothalamus is poorly characterized by proteomic strategies, we performed experiments aimed at constructing a two-dimensional gel electrophoresis (2-DE) profile of rat hypothalamus proteins.Results: As a first step, we established the best conditions for tissue collection and protein extraction, quantification and separation. the extraction buffer composition selected for proteome characterization of rat hypothalamus was urea 7 M, thiourea 2 M, CHAPS 4%, Triton X-100 0.5%, followed by a precipitation step with chloroform/methanol. Two-dimensional (2-D) gels of hypothalamic extracts from four-month-old rats were analyzed; the protein spots were digested and identified by using tandem mass spectrometry and database query using the protein search engine MASCOT. Eighty-six hypothalamic proteins were identified, the majority of which were classified as participating in metabolic processes, consistent with the finding of a large number of proteins with catalytic activity. Genes encoding proteins identified in this study have been related to obesity development.Conclusion: the present results indicate that the 2-DE technique will be useful for nutritional studies focusing on hypothalamic proteins. the data presented herein will serve as a reference database for studies testing the effects of dietary manipulations on hypothalamic proteome. We trust that these experiments will lead to important knowledge on protein targets of nutritional variables potentially able to affect the complex central nervous system control of energy homeostasis.en
dc.description.affiliationUniversidade Federal de São Paulo, Div Nutr Physiol, Dept Physiol, UNIFESP, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol Sci, UNIFESP, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Med, UNIFESP, Div Nephrol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, UNIFESP, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, FMRP, Ctr Quim Prot, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Fisiol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Nutr Physiol, Dept Physiol, UNIFESP, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biol Sci, UNIFESP, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Med, UNIFESP, Div Nephrol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, UNIFESP, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Fisiol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt
dc.format.extent15
dc.identifierhttps://dx.doi.org/10.1186/1477-5956-10-26
dc.identifier.citationProteome Science. London: Biomed Central Ltd, v. 10, 15 p., 2012.
dc.identifier.doi10.1186/1477-5956-10-26
dc.identifier.fileWOS000308914400001.pdf
dc.identifier.issn1477-5956
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/34801
dc.identifier.wosWOS:000308914400001
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofProteome Science
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHypothalamusen
dc.subjectProteomeen
dc.subject2-D electrophoresisen
dc.subjectMass spectrometryen
dc.subjectNutritionen
dc.subjectObesityen
dc.subjectRaten
dc.titleProteomic profiling of the rat hypothalamusen
dc.typeinfo:eu-repo/semantics/article
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