Comparative mRNA and MicroRNA Profiling during Acute Myocardial Infarction Induced by Coronary Occlusion and Ablation Radio-Frequency Currents

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dc.citation.volume7
dc.contributor.authorSantana, Eduardo T.
dc.contributor.authorFeliciano, Regiane dos Santos
dc.contributor.authorSerra, Andrey J.
dc.contributor.authorBrigidio, Eduardo
dc.contributor.authorAntonio, Ednei Luiz [UNIFESP]
dc.contributor.authorTucci, Paulo José Ferreira [UNIFESP]
dc.contributor.authorNathanson, Lubov
dc.contributor.authorMorris, Mariana
dc.contributor.authorSilva, Jose A., Jr.
dc.coverageLausanne
dc.date.accessioned2020-07-31T12:47:15Z
dc.date.available2020-07-31T12:47:15Z
dc.date.issued2016
dc.description.abstractThe ligation of the left anterior descending coronary artery is the most commonly used experimental model to induce myocardial infarction (MI) in rodents. A high mortality in the acute phase and the heterogeneity of the size of the MI obtained are drawbacks recognized in this model. In an attempt to solve the problem, our group recently developed a new MI experimental model which is based on application of myocardial ablation radio-frequency currents (AB-RF) that yielded MI with homogeneous sizes and significantly reduce acute mortality. In addition, cardiac structural, and functional changes aroused by AB-RF were similar to those seen in animals with MI induced by coronary artery ligation. Herein, we compared mRNA expression of genes that govern post-MI milieu in occlusion and ablation models. We analyzed 48 mRNAs expressions of nine different signal transduction pathways (cell survival and metabolism signs, matrix extracellular, cell cycle, oxidative stress, apoptosis, calcium signaling, hypertrophy markers, angiogenesis, and inflammation) in rat left ventricle 1 week after MI generated by both coronary occlusion and AB-RF. Furthermore, high-throughput miRNA analysis was also assessed in both MI procedures. Interestingly, mRNA expression levels and miRNA expressions showed strong similarities between both models after MI, with few specificities in each model, activating similar signal transduction pathways. To our knowledge, this is the first comparison of genomic alterations of mRNA and miRNA contents after two different MI procedures and identifies key signaling regulators modulating the pathophysiology of these two models that might culminate in heart failure. Furthermore, these analyses may contribute with the current knowledge concerning transcriptional and post-transcriptional changes of AB-RF protocol, arising as an alternative and effective MI method that reproduces most changes seem in coronary occlusion.en
dc.description.affiliationUniv Nove Julho, Rehabil Dept, Sao Paulo, Brazil
dc.description.affiliationUniv Nove Julho, Biophoton Dept, Sao Paulo, Brazil
dc.description.affiliationUniv Nove Julho, Dept Med, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Cardiac Physiol Dept, Sao Paulo, Brazil
dc.description.affiliationNova Southeastern Univ, Inst Neuroimmune Med, Ft Lauderdale, FL USA
dc.description.affiliationUnifespCardiac Physiology Department, Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2009/54225-8
dc.description.sponsorshipIDFAPESP: 2012/15808-0
dc.description.sponsorshipIDFAPESP: 2015/11028-9
dc.description.sponsorshipIDCNPq: 477458/2009-2
dc.description.sponsorshipIDCNPq: 479395/2012-8
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.3389/fphys.2016.00565
dc.identifier.citationFrontiers In Physiology. Lausanne, v. 7, p. -, 2016.
dc.identifier.doi10.3389/fphys.2016.00565
dc.identifier.fileWOS000388530000001.pdf
dc.identifier.issn1664-042X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56701
dc.identifier.wosWOS:000388530000001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Physiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectmyocardial infarctionen
dc.subjectRNAen
dc.subjectmicroRNAsen
dc.subjectratsen
dc.subjectWistaren
dc.subjectmethodsen
dc.titleComparative mRNA and MicroRNA Profiling during Acute Myocardial Infarction Induced by Coronary Occlusion and Ablation Radio-Frequency Currentsen
dc.typeinfo:eu-repo/semantics/article
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