LOW DIHYDROPYRIDINE RECEPTOR DENSITY IN VASA DEFERENTIA OF CASTRATED RATS

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dc.contributor.authorCastillo, CJF
dc.contributor.authorLafayette, Simone Sette Lopes [UNIFESP]
dc.contributor.authorCaricati-Neto, Afonso [UNIFESP]
dc.contributor.authorSette, Mario [UNIFESP]
dc.contributor.authorJurkiewicz, Neide Hyppolito [UNIFESP]
dc.contributor.authorGarcia, Antonio G.
dc.contributor.authorJurkiewicz, Aron [UNIFESP].
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUNIV AUTONOMA MADRID
dc.date.accessioned2018-06-15T16:24:07Z
dc.date.available2018-06-15T16:24:07Z
dc.date.issued1992-02-01
dc.description.abstractRadioligand binding studies in crude membrane preparations of vasa deferentia of normal rats, with the 1,4-dihydropyridine (+)-[H-3]-PN200-110 (isradipine) showed typical saturation isotherms. The binding exhibited a K(D) of 259 +/- 60 pM and B(max) of 144 +/- 20 fmol mg-1 protein. The low K(D) and the stereoselective displacement of (+)-[H-3]-PN200-110 binding by (+)- and (-)-PN200-110 and by nifedipine suggests that these tissues contain dihydropyridine receptors probably coupled to voltage-sensitive, L-type calcium channels. In membrane preparations from vasa deferentia from rats castrated 30 days previously the maximum specific binding was 25 +/- 10 fmol mg-1 protein, representing only 11% of total binding; thus, the calculation of reliable K(D) values was not feasible. These findings suggest that a testicular hormone, possibly testosterone, plays an important role in the regulation of dihydropyridine-sensitive, voltage-dependent calcium channels in the rat vas deferens.en
dc.description.affiliationESCOLA PAULISTA MED SCH,DEPT PHARMACOL,CAIXA POSTAL 20372,BR-04034 SAO PAULO,BRAZIL
dc.description.affiliationUNIV AUTONOMA MADRID,FAC MED,DEPT BIOCHEM,MADRID 34,SPAIN
dc.description.affiliationUNIV AUTONOMA MADRID,FAC MED,DEPT PHARMACOL,MADRID 34,SPAIN
dc.description.affiliationUnifespESCOLA PAULISTA MED SCH,DEPT PHARMACOL,CAIXA POSTAL 20372,BR-04034 SAO PAULO,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent257-258
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1111/j.1476-5381.1992.tb14241.x/full
dc.identifier.citationBritish Journal Of Pharmacology. Basingstoke: Stockton Press, v. 105, n. 2, p. 257-258, 1992.
dc.identifier.issn0007-1188
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43174
dc.identifier.wosWOS:A1992HC75900001
dc.language.isoeng
dc.publisherStockton Press
dc.relation.ispartofBritish Journal Of Pharmacology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDIHYDROPYRIDINE RECEPTORSen
dc.subjectVAS DEFERENSen
dc.subjectCALCIUM CHANNELSen
dc.subjectCASTRATIONen
dc.titleLOW DIHYDROPYRIDINE RECEPTOR DENSITY IN VASA DEFERENTIA OF CASTRATED RATSen
dc.typeinfo:eu-repo/semantics/article
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