Purification and biochemical characterization of an extracellular serine peptidase from aspergillus terreus

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dc.contributor.authorBiaggio, Rafael Tage
dc.contributor.authorda Silva, Ronivaldo Rodrigues
dc.contributor.authorda Rosa, Nathalia Gonsales
dc.contributor.authorRibeiro Leite, Rodrigo Simoes
dc.contributor.authorArantes, Eliane Candiani
dc.contributor.authorde Freitas Cabral, Tatiana Pereira
dc.contributor.authorJuliano, Maria A. [UNIFESP]
dc.contributor.authorJuliano, Luiz [UNIFESP]
dc.contributor.authorCabral, Hamilton
dc.date.accessioned2019-01-21T10:30:00Z
dc.date.available2019-01-21T10:30:00Z
dc.date.issued2016
dc.description.abstractPeptidases are important because they play a central role in pharmaceutical, food, environmental, and other industrial processes. A serine peptidase from Aspergillus terreus was isolated after two chromatography steps that showed a yield of 15.5%. Its molecular mass was determined to be 43 kD, by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). This peptidase was active between pH 5.0 to 8.0 and had maximum activity at pH 7.0, at 45 degrees C. When exposited with 1 M of urea, the enzyme maintained 100% activity and used azocasein as substrate. The N-terminal (first 15 residues) showed 33% identity with the serine peptidase of Aspergillus clavatus ES1. The kinetics assays showed that subsite S-2 did not bind polar basic amino acids (His and Arg) nonpolar acidic amino acids (Asp and Glu). The subsite S-1 showed higher catalytic efficiency than the S-2 and S-3 subsites.en
dc.description.affiliationDepartment of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences of Ribeirão Preto , University of São Paulo , Ribeirão Preto , Brazil
dc.description.affiliationInstitute of Biosciences, Letters and Exact Sciences , Universidade Estadual Paulista , São José do Rio Preto , Brazil
dc.description.affiliationFaculty of Biological and Environmental Sciences , Federal University of Grande Dourados , Dourados , Brazil
dc.description.affiliationDepartment of Physics and Chemistry, Faculty of Pharmaceutical Sciences of Ribeirão Preto , University of São Paulo , Ribeirão Preto , Brazil
dc.description.affiliationFaculty of Pharmacy , University Center of Educational Foundation of Barretos , Barretos , Brazil
dc.description.affiliationDepartment of Biophysics, Paulista School of Medicine , Federal University of São Paulo , São Paulo , Brazil
dc.description.affiliationUnifespDepartment of Biophysics, Paulista School of Medicine , Federal University of São Paulo , São Paulo , Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/06986-0, 2012/24703-8]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [308078/2012-8]
dc.description.sponsorshipIDFAPESP: 2011/06986-0
dc.description.sponsorshipIDFAPESP: 2012/24703-8
dc.description.sponsorshipIDCNPq: 308078/2012-8
dc.format.extent298-304
dc.identifierhttps://doi.org/10.1080/10826068.2015.1031387
dc.identifier.citationPreparative Biochemistry & Biotechnology. Philadelphia, v. 46, n. 3, p. 298-304, 2016.
dc.identifier.doi10.1080/10826068.2015.1031387
dc.identifier.issn1082-6068
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49527
dc.identifier.wosWOS:000374887000012
dc.language.isoeng
dc.publisherTaylor & francis inc
dc.relation.ispartofPreparative Biochemistry & Biotechnology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAspergillusen
dc.subjectEnzyme Kineticsen
dc.subjectFilamentous Fungien
dc.subjectN-Terminal Sequenceen
dc.subjectProteaseen
dc.subjectProteinMicrobial Alkaline Proteasesen
dc.subjectProteinasesen
dc.subjectSpecificityen
dc.subjectClavatusen
dc.titlePurification and biochemical characterization of an extracellular serine peptidase from aspergillus terreusen
dc.typeinfo:eu-repo/semantics/article
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