Manganese-enhanced magnetic resonance imaging detects mossy fiber sprouting in the pilocarpine model of epilepsy

dc.contributor.authorMalheiros, Jackeline M. [UNIFESP]
dc.contributor.authorPolli, Roberson S. [UNIFESP]
dc.contributor.authorPaiva, Fernando F.
dc.contributor.authorLongo, Beatriz M. [UNIFESP]
dc.contributor.authorMello, Luiz E. [UNIFESP]
dc.contributor.authorSilva, Afonso C.
dc.contributor.authorTannus, Alberto
dc.contributor.authorCovolan, Luciene [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionNatl Inst Neurol Disorders & Stroke
dc.description.abstractPurpose: Mossy fiber sprouting (MFS) is a frequent finding following status epilepticus (SE). the present study aimed to test the feasibility of using manganese-enhanced magnetic resonance imaging (MEMRI) to detect MFS in the chronic phase of the well-established pilocarpine (Pilo) rat model of temporal lobe epilepsy (TLE). Methods: To modulate MFS, cycloheximide (CHX), a protein synthesis inhibitor, was coadministered with Pilo in a subgroup of animals. in vivo MEMRI was performed 3 months after induction of SE and compared to the neo-Timm histologic labeling of zinc mossy fiber terminals in the dentate gyrus (DG). Key Findings: Chronically epileptic rats displaying MFS as detected by neo-Timm histology had a hyperintense MEMRI signal in the DG, whereas chronically epileptic animals that did not display MFS had minimal MEMRI signal enhancement compared to nonepileptic control animals. A strong correlation (r = 0.81, p < 0.001) was found between MEMRI signal enhancement and MFS. Significance: This study shows that MEMRI is an attractive noninvasive method for detection of mossy fiber sprouting in vivo and can be used as an evaluation tool in testing therapeutic approaches to manage chronic epilepsy.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Physiol, UNIFESP, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, CIERMag Sao Carlos Inst Phys, Sao Carlos, SP, Brazil
dc.description.affiliationNatl Inst Neurol Disorders & Stroke, Cerebral Microcirculat Unit, Lab Funct & Mol Imaging, NIH, Bethesda, MD USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Physiol, UNIFESP, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipNIH, NINDS
dc.description.sponsorshipIDFAPESP: 2005/56663-1
dc.description.sponsorshipIDFAPESP: 07/52911-6
dc.identifier.citationEpilepsia. Hoboken: Wiley-Blackwell, v. 53, n. 7, p. 1225-1232, 2012.
dc.rightsAcesso aberto
dc.subjectMossy fiber sproutingen
dc.subjectMagnetic resonance imagingen
dc.subjectAnimal modelsen
dc.titleManganese-enhanced magnetic resonance imaging detects mossy fiber sprouting in the pilocarpine model of epilepsyen