Tridimensional ultrastructure and glycolipid pattern studies of Trypanosoma dionisii

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dc.contributor.authorOliveira, Miriam Pires de Castro [UNIFESP]
dc.contributor.authorRamos, Thiago Cesar Prata [UNIFESP]
dc.contributor.authorPinheiro, Adriana Maria Viana Nunes [UNIFESP]
dc.contributor.authorBertini, Silvio [UNIFESP]
dc.contributor.authorTakahashi, Helio Kiyoshi [UNIFESP]
dc.contributor.authorStraus, Anita Hilda [UNIFESP]
dc.contributor.authorFreymüller-Haapalainen, Edna [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:34:44Z
dc.date.available2016-01-24T14:34:44Z
dc.date.issued2013-12-01
dc.description.abstractTrypanosoma (Schizotrypanum) dionisii is a non-pathogenic bat trypanosome closely related to Trypanosoma cruzi, the etiological agent of Chaga's disease. Both kinetoplastids present similar morphological stages and are able to infect mammalian cells in culture. in the present study we examined 3D ultrastructure aspects of the two species by serial sectioning epimastigote and trypomastigote forms, and identified common carbohydrate epitopes expressed in T. dionisii, T. cruzi and Leishmania major. A major difference in 3D morphology was that T. dionisii epimastigote forms present larger multivesicular structures, restricted to the parasite posterior region. These structures could be related to T. cruzi reservosomes and are also rich in cruzipain, the major cysteine-proteinase of T. cruzi. We analyzed the reactivity of two monoclonal antibodies: MEST-1 directed to galactofuranose residues of glycolipids purified from Paracoccidioides brasiliensis, and BST-1 directed to glycolipids purified from T. cruzi epimastigotes. Both antibodies were reactive with T. dionisii epimastigotes by indirect immunofluorescense, but we noted differences in the location and intensity of the epitopes, when compared to T. cruzi. in summary, despite similar features in cellular structure and life cycle of T. dionisii and T. cruzi, we observed a unique morphological characteristic in T. dionisii that deserves to be explored. (C) 2013 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol Estrutural & Func, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Ctr Microscopia Eletron, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, EPM, Dept Biol Estrutural & Func, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, EPM, Dept Bioquim, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, EPM, Ctr Microscopia Eletron, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 06/07005-4
dc.format.extent548-556
dc.identifierhttp://dx.doi.org/10.1016/j.actatropica.2013.08.001
dc.identifier.citationActa Tropica. Amsterdam: Elsevier B.V., v. 128, n. 3, p. 548-556, 2013.
dc.identifier.doi10.1016/j.actatropica.2013.08.001
dc.identifier.fileWOS000327824800015.pdf
dc.identifier.issn0001-706X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36993
dc.identifier.wosWOS:000327824800015
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofActa Tropica
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectTtypanosoma dionisiien
dc.subjectTridimensional ultrastructureen
dc.subject3D reconstructionen
dc.subjectEpimastigoteen
dc.subjectGlycolipiden
dc.titleTridimensional ultrastructure and glycolipid pattern studies of Trypanosoma dionisiien
dc.typeinfo:eu-repo/semantics/article
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