Prognostic significance of TIMP3 hypermethylation in post-treatment salivary rinse from head and neck squamous cell carcinoma patients

dc.contributor.authorRettori, Marianna Marconato [UNIFESP]
dc.contributor.authorCarvalho, Ana Carolina de [UNIFESP]
dc.contributor.authorBomfim Longo, Ana Luiza [UNIFESP]
dc.contributor.authorOliveira, Cleyton Zanardo de
dc.contributor.authorKowalski, Luiz Paulo
dc.contributor.authorCarvalho, Andre Lopes
dc.contributor.authorVettore, Andre Luiz [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionBarretos Canc Hosp
dc.contributor.institutionAC Camargo Canc Hosp
dc.date.accessioned2016-01-24T14:30:50Z
dc.date.available2016-01-24T14:30:50Z
dc.date.issued2013-01-01
dc.description.abstractHypermethylation in the promoter regions of genes is associated with suppression of gene expression and has been considered a potential molecular marker for several tumor types, including head and neck squamous cell carcinomas (HNSCC). Moreover, hypermethylation can be detected in body fluids such as saliva and can be useful for the diagnosis and prognosis of patients suffering from cancer. To evaluate the hypermethylation profile as a tool for early detection of tumor recurrences, this study determines the methylation status of 24 genes in salivary rinses collected from HNSCC patients at diagnosis, just after the last curative treatment and in the patients' follow-up visit at 6 months after treatment. in the analysis of salivary rinse samples taken at diagnosis of HNSCC patients, five genes (CCNA1, DAPK, DCC, MGMT and TIMP3) showed high specificity and sensitivity. Hypermethylation in any of these five genes was correlated with the presence of tumors in the oral cavity. Patients with TIMP3 methylation in samples collected 6 months after the last curative treatment had lower local recurrence-free survival (P 0.008). Multivariate analysis confirmed that this hypermethylation pattern remained as an independent prognostic factor for local recurrence (P 0.025). This study presents, for the first time, the detection of TIMP3 promoter hypermethylation in post-treatment salivary rinse as an independent prognostic maker for local recurrence-free survival in patients with HNSCC, justifying the use of DNA hypermethylation detection in saliva as a tool for identifying and monitoring HNSCC patients' subgroups with high risk of developing local recurrence.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol Sci, BR-04039032 São Paulo, Brazil
dc.description.affiliationBarretos Canc Hosp, Stat & Epidemiol Ctr, BR-14784400 Barretos, Brazil
dc.description.affiliationAC Camargo Canc Hosp, Dept Head & Neck Surg, BR-01509010 São Paulo, Brazil
dc.description.affiliationBarretos Canc Hosp, Dept Head & Neck Surg, BR-14784400 Barretos, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biol Sci, BR-04039032 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2008/58460-9
dc.description.sponsorshipIDFAPESP: 2005/02580-8
dc.description.sponsorshipIDFAPESP: 07/56245-0
dc.description.sponsorshipIDFAPESP: 07/56471-0
dc.description.sponsorshipIDCNPq: 313181/2009-8
dc.description.sponsorshipIDCNPq: 302360/2008-5
dc.format.extent20-27
dc.identifierhttp://dx.doi.org/10.1093/carcin/bgs311
dc.identifier.citationCarcinogenesis. Oxford: Oxford Univ Press, v. 34, n. 1, p. 20-27, 2013.
dc.identifier.doi10.1093/carcin/bgs311
dc.identifier.issn0143-3334
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35660
dc.identifier.wosWOS:000313128600004
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofCarcinogenesis
dc.rightsAcesso aberto
dc.rights.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.titlePrognostic significance of TIMP3 hypermethylation in post-treatment salivary rinse from head and neck squamous cell carcinoma patientsen
dc.typeArtigo
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