Human neutrophil migration in vitro induced by secretory phospholipases A(2): a role for cell surface glycosaminoglycans

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dc.contributor.authorGambero, A.
dc.contributor.authorLanducci, ECT
dc.contributor.authorToyama, M. H.
dc.contributor.authorMarangoni, S.
dc.contributor.authorGiglio, JR
dc.contributor.authorNader, H. B.
dc.contributor.authorDietrich, C. P.
dc.contributor.authorDe Nucci, G.
dc.contributor.authorAntunes, E.
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:33:11Z
dc.date.available2016-01-24T12:33:11Z
dc.date.issued2002-01-01
dc.description.abstractThe purpose of this study was to examine the ability of type I- (porcine pancreas and Naja mocambique mocambique venom), type II- (bothropstoxin-I, bothropstoxin-II, and piratoxin-I). and type III- (Apis mellifera venom) secretory phospholipases A(2) (sPLA(2)s) to induce human neutrophil chemotaxis, and the role of the cell surface proteoglycans, leukotriene B-4 (LTB4), and platelet-activating factor (PAF). in mediating this migration. the neutrophil chemotaxis assays were performed by using a 48-well microchemotaxis chamber. Piratoxin-I, bothropstoxin-I. N. m. mocambique venom PLA(2) (10-1000 mug/mL each), bothropstoxin-II (30-1000 mug/mL), porcine pancreas PLA(2) (0.3-30 mug/mL), and A. mellifera venom PLA(2) (30-300 mug/mL) caused concentration-dependent neutrophil chemotaxis. Heparin (10-300 U/mL) concentration-dependently inhibited the neutrophil migration induced by piratoxin-I, bothropstoxin-II, and N. in. mocambique and A. mellifera venom PLA(2)s (100 mug/mL each), but failed to affect the migration induced by porcine pancreas PLA(2). Heparan sulfate (300 and 1000 mug/mL) inhibited neutrophil migration induced by piratoxin-I, whereas dermatan sulfate and chondroitin sulfate (30-1000 mug/mL each) had no effect. Heparitinase I and heparinase (300 mU/mL each) inhibited by 41.5 and 47%. respectively, piratoxin-l-induced chemotaxis, whereas heparitinase 11 and chondroitinase AC failed to affect the chemotaxis. the PAF receptor antagonist WEB 2086 (3-[4-(2-chlorophenyl)-9-methyl-6H-thienol-[3,2-f] [1,2,4]-triazolo-[4.3-a] [1,4]-diazepine-2-yl]-1-(4-morpholynil)-1-propionate) (0.1-10 muM) and the LTB4 synthesis inhibitor AA-861 [2-(12-hydroxydodeca-5,10-diynyl)-3,5,6-trimethyl-1,4-benzoquinone] (0.1-10 muM) significantly inhibited the piratoxin-I-induced chemotaxis. Piratoxin-I (30-300 mug/mL) caused a concentration-dependent release of LTB4 Our results suggest that neutrophil migration in response to sPLA(2)s is independent of PLA activity, and involves an interaction of sPLA(2)s with cell surface heparin/heparan binding sites triggering the release of LTB4 and PAF. (C) 2002 Elsevier Science Inc. All rights reserved.en
dc.description.affiliationUNICAMP, Dept Pharmacol, Fac Med Sci, BR-13081970 Campinas, SP, Brazil
dc.description.affiliationUNICAMP, Dept Biochem, BR-13081970 Campinas, SP, Brazil
dc.description.affiliationUSP, Dept Biochem, Ribeirao Preto, Brazil
dc.description.affiliationUNIFESP, Dept Biochem, São Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Dept Biochem, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent65-72
dc.identifierhttp://dx.doi.org/10.1016/S0006-2952(01)00841-3
dc.identifier.citationBiochemical Pharmacology. Oxford: Pergamon-Elsevier B.V., v. 63, n. 1, p. 65-72, 2002.
dc.identifier.doi10.1016/S0006-2952(01)00841-3
dc.identifier.issn0006-2952
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26696
dc.identifier.wosWOS:000173153700008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochemical Pharmacology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectphospholipase A(2)en
dc.subjectneutrophil chemotaxisen
dc.subjectFlavobacterium heparinum lyasesen
dc.subjectleukotriene B-4en
dc.subjectplatelet-activating factoren
dc.subjectN-formyl-methionylleucyl-phenylalanineen
dc.titleHuman neutrophil migration in vitro induced by secretory phospholipases A(2): a role for cell surface glycosaminoglycansen
dc.typeinfo:eu-repo/semantics/article
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