Amifostine protective effect on cisplatin-treated rat testis

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dc.contributor.authorLirdi, Leandra Campos [UNIFESP]
dc.contributor.authorStumpp, Taiza [UNIFESP]
dc.contributor.authorSasso-Cerri, Estela
dc.contributor.authorMiraglia, Sandra Maria [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionSão Paulo State Univ
dc.date.accessioned2016-01-24T13:51:29Z
dc.date.available2016-01-24T13:51:29Z
dc.date.issued2008-07-01
dc.description.abstractCisplatin is a potent drug used in clinical oncology but causes spermatogenesis damage. Amifostine is a drug used against toxicity caused by ionizing irradiation and chemotherapeutic drugs. Since cisplatin provokes fertility and induces germ cell apoptosis and necrosis, we proposed to evaluate the amifostine cytoprotective action on testes of cisplatin-treated rats. Thirty-day-old prepubertal Wistar rats received a single cisplatin dose of 5 mg/kg and were killed after 3, 6, and 12 hr. the hematoxylin-eosin stained testicular sections were submitted to histological, morphometric, and stereological analysis. the terminal deoxynucleotidyl transferase-mediated deoxyuridinetriphosphate nick end-labeling (TUNEL) method was used to label apoptotic cells. TUNEL-positive and TUNEL-negative germ cells with abnormal nuclear morphology (ANM) were scored. Significant alterations of greater part of the parameters occurred in the cisplatin-treated group (CE) compared to the group that received amifostine before the cisplatin-treatment (ACE); however, testicular weight and volume did not vary between these groups. Tubular diameter was reduced in CE in comparison to ACE rats, while interstitial tissue and lymphatic space volume and volume density were significantly higher in CE rats; interstitial testicular edema probably occurred in cisplatin-treated rats. CE rats showed important histological alterations, which were more accentuated than in ACE rats. the numerical densities of apoptotic germ cells and TUNEL-negative cells with ANM were lower in ACE than in CE rats. in conclusion, the amifostine previously administered to prepubertal rats reduced the testicular damage caused by cisplatin. We conclude that amifostine partially protected the rat seminiferous epithelium against cisplatin toxicity.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morphol & Genet, BR-04023900 São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Dept Morphol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morphol & Genet, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent797-808
dc.identifierhttp://dx.doi.org/10.1002/ar.20693
dc.identifier.citationAnatomical Record-advances in Integrative Anatomy and Evolutionary Biology. Hoboken: Wiley-liss, v. 291, n. 7, p. 797-808, 2008.
dc.identifier.doi10.1002/ar.20693
dc.identifier.issn1932-8486
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30738
dc.identifier.wosWOS:000257333600007
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofAnatomical Record-advances in Integrative Anatomy and Evolutionary Biology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectamifostineen
dc.subjectcisplatinen
dc.subjectapoptosisen
dc.subjecttestisen
dc.subjectratsen
dc.titleAmifostine protective effect on cisplatin-treated rat testisen
dc.typeinfo:eu-repo/semantics/article
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