Different patterns of neuronal activation and neurodegeneration in the thalamus and cortex of epilepsy-resistant Proechimys rats versus Wistar rats after pilocarpine-induced protracted seizures

dc.contributor.authorAndrioli, Anna
dc.contributor.authorFabene, Paolo F.
dc.contributor.authorSpreafico, Roberto
dc.contributor.authorCavalheiro, Esper Abrão [UNIFESP]
dc.contributor.authorBentivoglio, Marina
dc.contributor.institutionUniv Verona
dc.contributor.institutionNeurol Inst Carlo Besta
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:52:22Z
dc.date.available2016-01-24T13:52:22Z
dc.date.issued2009-04-01
dc.description.abstractTo analyze cellular mechanisms of limbic-seizure suppression, the response to pilocarpine-induced seizures was investigated in cortex and thalamus, comparing epilepsy-resistant rats Proechimys guyannensis with Wistar rats.Fos immunoreactivity revealing neuronal activation, and degenerating neurons labeled by Fluoro-Jade B (FJB) histochemistry were analyzed on the first day after onset of seizures lasting 3 h. Subpopulations of gamma-aminobutyric acid (GABA)ergic cells were characterized with double Fos-parvalbumin immunohistochemistry.In both cortex and thalamus, degenerating neurons were much fewer in Proechimys than Wistar rats. Fos persisted at high levels at 24 h only in the Proechimys thalamus and cortex, especially in layer VI where corticothalamic neurons reside. in the parietal cortex, about 50% of parvalbumin-containing interneurons at 8 h, and 10-20% at 24 h, were Fos-positive in Wistar rats, but in Proechimys, Fos was expressed in almost all parvalbumin-containing interneurons at 8 h and dropped at 24 h. Fos positivity in cingulate cortex interneurons was similar in both species. in the Wistar rat thalamus, Fos was induced in medial and midline nuclei up to 8 h, when < 30% of reticular nucleus cells were Fos-positive, and then decreased, with no relationship with cell loss, evaluated in Nissl-stained sections. in Proechimys, almost all reticular nucleus neurons were Fos-positive at 24 h.At variance with laboratory rats, pilocarpine-induced protracted seizures elicit in Proechimys limited neuronal death, and marked and long-lasting Fos induction in excitatory and inhibitory cortical and thalamic cell subsets. the findings implicate intrathalamic and intracortical regulation, and circuits linking thalamus and cortex in limbic seizure suppression leading to epilepsy resistance.en
dc.description.affiliationUniv Verona, Fac Med, Dept Morphol & Biomed Sci, I-37134 Verona, Italy
dc.description.affiliationNeurol Inst Carlo Besta, Milan, Italy
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Expt Neurol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Expt Neurol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent832-848
dc.identifierhttp://dx.doi.org/10.1111/j.1528-1167.2008.01953.x
dc.identifier.citationEpilepsia. Malden: Wiley-Blackwell Publishing, Inc, v. 50, n. 4, p. 832-848, 2009.
dc.identifier.doi10.1111/j.1528-1167.2008.01953.x
dc.identifier.issn0013-9580
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31398
dc.identifier.wosWOS:000264821700025
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofEpilepsia
dc.rightsAcesso aberto
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectFosen
dc.subjectCell deathen
dc.subjectThalamic reticular nucleusen
dc.subjectCorticothalamic systemen
dc.subjectCingulate cortexen
dc.subjectNeocortexen
dc.titleDifferent patterns of neuronal activation and neurodegeneration in the thalamus and cortex of epilepsy-resistant Proechimys rats versus Wistar rats after pilocarpine-induced protracted seizuresen
dc.typeArtigo
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