Effect of Concentrated Apple Extract on Experimental Colitis Induced by Acetic Acid

Página do item simplificado
dc.citation.issue1
dc.citation.volume6
dc.contributor.authorPastrelo, Maurcio Mercaldi [UNIFESP]
dc.contributor.authorRibeiro, Carla Caroline Dias [UNIFESP]
dc.contributor.authorDuarte, Joselmo Willamys [UNIFESP]
dc.contributor.authorGollucke, Andrea Pittelli Boiago [UNIFESP]
dc.contributor.authorArtigiani Neto, Ricardo [UNIFESP]
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorMiszputen, Sender Jankiel [UNIFESP]
dc.contributor.authorOshima, Celina Tizuko Fujiyama [UNIFESP]
dc.contributor.authorPaiotti, Ana Paula Ribeiro [UNIFESP]
dc.coverageBabol
dc.date.accessioned2020-09-01T13:21:15Z
dc.date.available2020-09-01T13:21:15Z
dc.date.issued2017
dc.description.abstractReactive oxygen and nitrogen species (ROS/RNS) play a crucial role in inflammatory bowel disease (IBD) exacerbating the chronic inflammatory process. Endogenous and diet antioxidants can neutralize these compounds. The apple is widely consumed, with several antioxidant activity compounds. The present study evaluated the effects of concentrated apple extract (CAE) in acetic acid induced colitis. 29 Wistar male rats were randomized into 5 groups. G1-Sham/saline solution, G2-CAE/control, G3-acetic acid/control, G4-curative-CAE treatment and G5-preventive-CAE treatment. Eight days later, the animals were euthanized and the colonic segment resected for macroscopic and histological analysis. Gene expression was evaluated for inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), catalase and copper and zinc superoxide dismutase (CuZnSOD) by quantitative real time PCR, while protein expression was assessed for iNOS, COX-2 and 8-hydroxy-20-deoxyguanosine (8-OHdG) via immunohistochemistry. The groups G3, G4 and G5 had weight loss, while G5 had weight increase at the end of the experiment. The treatment with CAE reduced the macroscopic and microscopic injury, decreased iNOS mRNA expression and increased CuZnSOD mRNA expression in animals with induced acetic acid-colitis. The findings of the present study suggest that CAE treatment exerts an antioxidant role by downregulating iNOS and upregulating CuZnSOD.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Pathol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Discipline Gastroenterol, Dept Med, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biosci, Santos, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Pathol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Discipline Gastroenterol, Dept Med, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biosci, Santos, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2013/26845-7
dc.format.extent38-49
dc.identifierhttps://dx.doi.org/10.22088/acadpub.BUMS.6.1.38
dc.identifier.citationInternational Journal Of Molecular And Cellular Medicine. Babol, v. 6, n. 1, p. 38-49, 2017.
dc.identifier.doi10.22088/acadpub.BUMS.6.1.38
dc.identifier.issn2251-9637
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/58157
dc.identifier.wosWOS:000403138600005
dc.language.isoeng
dc.publisherCellular & Molecular Biology Research Center
dc.relation.ispartofInternational Journal Of Molecular And Cellular Medicine
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectExperimental colitisen
dc.subjectApple extracten
dc.subjectAntioxidanten
dc.subjectiNOSen
dc.subjectCuZnSODen
dc.titleEffect of Concentrated Apple Extract on Experimental Colitis Induced by Acetic Aciden
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos
ISS - Artigos