Molecular profiling of isolated histological components of Wilms tumor implicates a common role for the Wnt signaling pathway in kidney and tumor development

dc.contributor.authorMaschietto, Mariana
dc.contributor.authorCamargo, Beatriz de
dc.contributor.authorBrentani, Helena
dc.contributor.authorGrundy, Paul
dc.contributor.authorSredni, Simone T.
dc.contributor.authorTorres, Cesar
dc.contributor.authorMota, Louise D.
dc.contributor.authorCunha, Isabela W.
dc.contributor.authorPatrao, Diogo F. C.
dc.contributor.authorCosta, Cecilia M. L.
dc.contributor.authorSoares, Fernando A.
dc.contributor.authorBrentani, Ricardo R.
dc.contributor.authorCarraro, Dirce M.
dc.contributor.institutionHosp AC Camargo Fund Antonio Prudente
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Alberta
dc.contributor.institutionNorthwestern Univ
dc.date.accessioned2016-01-24T13:49:28Z
dc.date.available2016-01-24T13:49:28Z
dc.date.issued2008-01-01
dc.description.abstractWilms tumor (WT), a tumor composed of three histological components - blastema (BL), epithelia and stroma - is considered an appropriate model system to study the biological relationship between differentiation and tumorigenesis. To investigate molecular associations between nephrogenesis and WT, the gene expression pattern of individual cellular components was analyzed, using a customized platform containing 4,608 genes. WT gene expression patterns were compared to genes regulated during kidney differentiation. BL had a closer gene expression pattern to the earliest stage of normal renal development. the BL gene expression pattern was compared to that of fetal kidney (FK) and also between FK and mature kidney, identifying 25 common de-regulated genes supposedly involved in the earliest events of WT onset. Quantitative RT-PCR was performed, confirming the difference in expression levels for 13 of 16 genes (81.2%) in the initial set and 8 of 13 (61.5%) in an independent set of samples. An overrepresentation of genes belonging to the Wnt signaling pathway was identified, namely PLCG2, ROCK2 and adenomatous polyposis coli (APC). Activation of the Wnt pathway was confirmed in WT, using APC at protein level and PLCG2 at mRNA and protein level. APC showed positive nuclear immunostaining for an independent set of WT samples, similarly to the FK in week 11. Lack of PLCG2 expression was confirmed in WT and in FK until week 18. Taken together, these results provided molecular evidence of the recapitulation of the embryonic kidney by WT as well as involvement of the Wnt pathway in the earliest events of WT onset. Copyright (C) 2008 S. Karger AG, Basel.en
dc.description.affiliationHosp AC Camargo Fund Antonio Prudente, Lab Genom & Mol Biol, BR-01509010 São Paulo, Brazil
dc.description.affiliationHosp AC Camargo Fund Antonio Prudente, Lab Bioinformat, BR-01509010 São Paulo, Brazil
dc.description.affiliationHosp AC Camargo Fund Antonio Prudente, Dept Pediat, BR-01509010 São Paulo, Brazil
dc.description.affiliationHosp AC Camargo Fund Antonio Prudente, Dept Anat Pathol, BR-01509010 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Dept Oncol, BR-09500900 São Paulo, Brazil
dc.description.affiliationUniv Alberta, Dept Pediat & Oncol, Edmonton, AB, Canada
dc.description.affiliationNorthwestern Univ, Feinberg Sch Med, Childrens Mem Res Ctr, Chicago, IL 60611 USA
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 98/14335-2
dc.description.sponsorshipIDFAPESP: 06/00054-0
dc.description.sponsorshipIDFAPESP: 06/00081-7
dc.format.extent81-91
dc.identifierhttp://dx.doi.org/10.1159/000155210
dc.identifier.citationOncology. Basel: Karger, v. 75, n. 1-2, p. 81-91, 2008.
dc.identifier.doi10.1159/000155210
dc.identifier.issn0030-2414
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30359
dc.identifier.wosWOS:000259892700011
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofOncology
dc.rightsAcesso restrito
dc.rights.licensehttp://www.karger.com/Services/RightsPermissions
dc.subjectblastemal componenten
dc.subjectgene expression analysisen
dc.subjectkidney developmenten
dc.subjectWilms tumoren
dc.subjectWnt signaling pathwayen
dc.titleMolecular profiling of isolated histological components of Wilms tumor implicates a common role for the Wnt signaling pathway in kidney and tumor developmenten
dc.typeArtigo
Arquivos