Conservation of genetic linkage between heat shock protein 100 and glycosylphosphatidylinositol-specific phospholipase C in Trypanosoma brucei and Trypanosoma cruzi

dc.contributor.authorRedpath, M. B.
dc.contributor.authorCarnall, N.
dc.contributor.authorWebb, H.
dc.contributor.authorCourel, M.
dc.contributor.authorAmorim, A.
dc.contributor.authorGuther, MLS
dc.contributor.authorAlmeida, MLC de
dc.contributor.authorCarrington, M.
dc.contributor.institutionUniv Cambridge
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Dundee
dc.date.accessioned2016-01-24T12:30:37Z
dc.date.available2016-01-24T12:30:37Z
dc.date.issued1998-07-01
dc.description.abstractThe experiments described in this paper were designed to try and isolate a recombinant DNA clone encoding a Trypanosoma cruzi homologue of the Trypanosoma br brucei glycosylphosphatidylinositol-specific phospholipase C (GPI-PLC) gene. Despite the ready biochemical detection of phospholipase C activities that hydrolyse GPI-anchors of cell surface proteins in T. cruzi, it did not prove possible to isolate any recombinant DNA clones using the T. brucei gpi-plc gene as a probe. On determining the DNA sequence to the 5' side of the gpi-plc gene it was found to be adjacent to a gene that encodes a 100 kDa heat shock protein (HSP100). To investigate whether this linkage between the hsp100 and gpi-plc genes was conserved in T. cruzi, a probe derived from the T. brucei hsp100 gene was used to isolate T. cruzi genomic clones. These were partially sequenced and shown to contain an hsp100 gene. Restriction enzyme fragments located to the 3' side of the T. cruzi hsp100 gene were then sequenced and found to contain a gene that encodes a polypeptide (TcPLC1) that has 46% amino acid sequence identity with the T. brucei GPI-PLC including most of the key residues involved in inositol binding and the catalytic histidine. A recombinant form of TcPLC1 was produced and shown to possess phospholipase C activity towards a GPI-substrate. Thus, the hsp100 and gpi-plc genes are adjacent in T. brucei and this linkage is conserved in T. cruzi. This observation has been used to facilitate the isolation of a clone encoding a T. cruzi phospholipase C gene. (C) 1998 Published by Elsevier Science B.V. All rights reserved.en
dc.description.affiliationUniv Cambridge, Dept Biochem, Cambridge CB2 1GA, England
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Dundee, Dept Biochem, Dundee DD1 4HN, Scotland
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent113-121
dc.identifierhttp://dx.doi.org/10.1016/S0166-6851(98)00056-5
dc.identifier.citationMolecular and Biochemical Parasitology. Amsterdam: Elsevier B.V., v. 94, n. 1, p. 113-121, 1998.
dc.identifier.doi10.1016/S0166-6851(98)00056-5
dc.identifier.issn0166-6851
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/25926
dc.identifier.wosWOS:000075308600009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMolecular and Biochemical Parasitology
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectTrypanosoma bruceien
dc.subjectTrypanosoma cruzien
dc.subjectglycosylphosphatidylinositolen
dc.subjectphospholipase Cen
dc.subjectheat shocken
dc.subjectprotein 100en
dc.titleConservation of genetic linkage between heat shock protein 100 and glycosylphosphatidylinositol-specific phospholipase C in Trypanosoma brucei and Trypanosoma cruzien
dc.typeArtigo
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