Behavior of human immunoglobulin G adsorption onto immobilized Cu(II) affinity hollow-fiber membranes
| dc.contributor.author | Borsoi-Ribeiro, Mariana | |
| dc.contributor.author | Lazzarotto Bresolin, Igor Tadeu [UNIFESP] | |
| dc.contributor.author | Vijayalakshmi, Mookambeswaran | |
| dc.contributor.author | Alves Bueno, Sonia Maria | |
| dc.contributor.institution | Universidade Estadual de Campinas (UNICAMP) | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.contributor.institution | Univ Technol Compiegne | |
| dc.contributor.institution | VIT Univ Vellore | |
| dc.date.accessioned | 2016-01-24T14:34:28Z | |
| dc.date.available | 2016-01-24T14:34:28Z | |
| dc.date.issued | 2013-10-01 | |
| dc.description.abstract | Iminodiacetic acid (IDA) and tris(2-aminoethyl)amine (TREN) chelating ligands were immobilized on poly(ethylene vinyl alcohol) (PEVA) hollow-fiber membranes after activation with epichlorohydrin or butanediol diglycidyl ether (bisoxirane). the affinity membranes complexed with Cu(II) were evaluated for adsorption of human immunoglobulin G (IgG). the effects of matrix activation and buffer system on adsorption of IgG were studied. Isotherms of batch IgG adsorption onto finely cut membranes showed that neither of the chelates, IDA-Cu(II) or TREN-Cu(II), had a Langmuirean behavior with negative cooperativity for IgG binding. A comparison of equilibrium and dynamic maximum capacities showed that the dynamic capacity for a mini-cartridge in a cross-flow filtration mode (52.5 and 298.4mgg(-1) dry weight for PEVA-TREN-Cu(II) and PEVA-IDA-Cu(II), respectively) was somewhat higher than the equilibrium capacity (9.2 and 73.3mgg(-1) dry weight for PEVA-TREN-Cu(II) and PEVA-IDA-Cu(II), respectively). When mini-cartridges were used, the dynamic adsorption capacity of IDA-Cu(II) was the same for both mini-cartridge and agarose gel. Copyright (c) 2013 John Wiley & Sons, Ltd. | en |
| dc.description.affiliation | Univ Estadual Campinas, UNICAMP, Sch Chem Engn, BR-13083970 Campinas, SP, Brazil | |
| dc.description.affiliation | Universidade Federal de São Paulo, UNIFESP, Dept Exact & Earth Sci, BR-09913030 São Paulo, Brazil | |
| dc.description.affiliation | Univ Technol Compiegne, Lab Interact Mol & Technol Separat, F-60205 Compiegne, France | |
| dc.description.affiliation | VIT Univ Vellore, Ctr Bioseparat Technol, Vellore 632014, Tamil Nadu, India | |
| dc.description.affiliationUnifesp | Universidade Federal de São Paulo, UNIFESP, Dept Exact & Earth Sci, BR-09913030 São Paulo, Brazil | |
| dc.description.source | Web of Science | |
| dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
| dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
| dc.format.extent | 514-520 | |
| dc.identifier | http://dx.doi.org/10.1002/jmr.2296 | |
| dc.identifier.citation | Journal of Molecular Recognition. Hoboken: Wiley-Blackwell, v. 26, n. 10, p. 514-520, 2013. | |
| dc.identifier.doi | 10.1002/jmr.2296 | |
| dc.identifier.issn | 0952-3499 | |
| dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/36790 | |
| dc.identifier.wos | WOS:000323674900010 | |
| dc.language.iso | eng | |
| dc.publisher | Wiley-Blackwell | |
| dc.relation.ispartof | Journal of Molecular Recognition | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.license | http://olabout.wiley.com/WileyCDA/Section/id-406071.html | |
| dc.subject | human IgG | en |
| dc.subject | IMAC | en |
| dc.subject | Cu(II) | en |
| dc.subject | tris(2-aminoethyl)amine | en |
| dc.subject | affinity membranes | en |
| dc.subject | adsorption | en |
| dc.title | Behavior of human immunoglobulin G adsorption onto immobilized Cu(II) affinity hollow-fiber membranes | en |
| dc.type | info:eu-repo/semantics/article |