Quantification of angiogenesis induced in rabbit cornea by breast carcinoma of women treated with tamoxifen

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dc.contributor.authorDa Silva, B. B.
dc.contributor.authorDa Silva, R. G.
dc.contributor.authorBorges, U. S.
dc.contributor.authorFilho, MAGD
dc.contributor.authorPimentel, ICC
dc.contributor.authorGebrim, L. H.
dc.contributor.authorSimoes, M. D.
dc.contributor.authorBaracat, E. C.
dc.contributor.institutionFed Univ Piaui
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:37:49Z
dc.date.available2016-01-24T12:37:49Z
dc.date.issued2005-05-01
dc.description.abstractBackground: the aim of this study was to evaluate the effect of tamoxifen on angiogenesis induced in rabbit cornea by breast curcinoma from post-menopausal women.Methods: Thirteen post-menopausal women with operable, estrogen receptor-positive breast carcinomas, treated with 20 mg of tamoxifen daily for 30 days prior to undergoing definitive surgery, participated in this study. Twenty-six virgin female New Zealand white rabbits, 3-4 months old, weighing approximately 2.5 kg each, were divided into two groups of 13 animals and received corneal implants of tumor either pre- or post-tamoxifen treatment. After 10 days, tie animals were sacrificed, the region of the cornea between the tumor implant and the limbus was removed and quantification of angiogenesis was carried out by eualuating the entire hematoxylineosin stained slide, using a 10x objective lens (100x magnification). Student t-test was used in the statistical analysis of the data and statistical significance was established at P < 0.05.Results: Mean microvessel count was 106.8 +/- 5.9 pre-tamoxifen treatment and 54 +/- 5.6 post-treatment. According to Student t-test, there was a significant reduction in mean microvessel density following treatment with tamoxifen (P < 0.001).Conclusions: Rabbit cornea proved to be an interesing experimental model for the quantification of angiogenesis. Tamoxifen, when administered for 30 days to postmenopausal women with breast cancer, significantly inhibited angiogenesis induced by tumor fragments in rabbit cornea. (C) 2005 Wiley-Liss, Inc.en
dc.description.affiliationFed Univ Piaui, Dept Gynecol, Teresina, Piaui, Brazil
dc.description.affiliationFed Univ Piaui, Dept Pathol, Teresina, Piaui, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Gynecol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morphol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Gynecol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morphol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent77-80
dc.identifierhttp://dx.doi.org/10.1002/jso.20239
dc.identifier.citationJournal of Surgical Oncology. Hoboken: Wiley-liss, v. 90, n. 2, p. 77-80, 2005.
dc.identifier.doi10.1002/jso.20239
dc.identifier.issn0022-4790
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28271
dc.identifier.wosWOS:000228782800008
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Surgical Oncology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjecttamoxifenen
dc.subjectangiogenesisen
dc.subjectbreast carcinomaen
dc.subjectrabbit corneaen
dc.titleQuantification of angiogenesis induced in rabbit cornea by breast carcinoma of women treated with tamoxifenen
dc.typeinfo:eu-repo/semantics/article
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