Seasonal Variation, Chemical Composition and Antioxidant activity of Brazilian Propolis Samples

dc.contributor.authorTeixeira, Erica Weinstein
dc.contributor.authorMessage, Dejair
dc.contributor.authorNegri, Giuseppina [UNIFESP]
dc.contributor.authorSalatino, Antonio
dc.contributor.authorStringheta, Paulo Cesar
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de Viçosa (UFV)
dc.contributor.institutionAgencia Paulista Tecnol Agronegocios Secretaria A
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:05:21Z
dc.date.available2016-01-24T14:05:21Z
dc.date.issued2010-09-01
dc.description.abstractTotal phenolic contents, antioxidant activity and chemical composition of propolis samples from three localities of Minas Gerais state (southeast Brazil) were determined. Total phenolic contents were determined by the Folin-Ciocalteau method, antioxidant activity was evaluated by DPPH, using BHT as reference, and chemical composition was analyzed by GC/MS. Propolis from Itapecerica and Paula Candido municipalities were found to have high phenolic contents and pronounced antioxidant activity. From these extracts, 40 substances were identified, among them were simple phenylpropanoids, prenylated phenylpropanoids, sesqui- and diterpenoids. Quantitatively, the main constituent of both samples was allyl-3-prenylcinnamic acid. A sample from Virginopolis municipality had no detectable phenolic substances and contained mainly triterpenoids, the main constituents being alpha-and beta-amyrins. Methanolic extracts from Itapecerica and Paula Candido exhibited pronounced scavenging activity towards DPPH, indistinguishable from BHT activity. However, extracts from Virginopolis sample exhibited no antioxidant activity. Total phenolic substances, GC/MS analyses and antioxidant activity of samples from Itapecerica collected monthly over a period of 1 year revealed considerable variation. No correlation was observed between antioxidant activity and either total phenolic contents or contents of artepillin C and other phenolic substances, as assayed by CG/MS analysis.en
dc.description.affiliationUniv São Paulo, Inst Biosci, Dept Bot, BR-05422970 São Paulo, Brazil
dc.description.affiliationUniv Fed Vicosa, Dept Tecnol Alimentos, BR-36571000 Vicosa, MG, Brazil
dc.description.affiliationAgencia Paulista Tecnol Agronegocios Secretaria A, BR-12400970 Pindamonhangaba, SP, Brazil
dc.description.affiliationUniv Fed Vicosa, Dept Biol Anim, BR-36571000 Vicosa, MG, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Psicobiol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Psicobiol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent307-315
dc.identifierhttp://dx.doi.org/10.1093/ecam/nem177
dc.identifier.citationEvidence-based Complementary and Alternative Medicine. Oxford: Oxford Univ Press, v. 7, n. 3, p. 307-315, 2010.
dc.identifier.doi10.1093/ecam/nem177
dc.identifier.fileWOS000279347500006.pdf
dc.identifier.issn1741-427X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32839
dc.identifier.wosWOS:000279347500006
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofEvidence-based Complementary and Alternative Medicine
dc.rightsAcesso aberto
dc.rights.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.subjectantioxidant activityen
dc.subjectApis melliferaen
dc.subjectBaccharis dracunculifoliaen
dc.subjectDPPHen
dc.subjectpropolisen
dc.subjectseasonalityen
dc.titleSeasonal Variation, Chemical Composition and Antioxidant activity of Brazilian Propolis Samplesen
dc.typeArtigo
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