Effects of ethanol on hippocampal neurogenesis depend on the conditioned appetitive response

dc.contributor.authorTesone-Coelho, Carolina [UNIFESP]
dc.contributor.authorVarela, Patricia [UNIFESP]
dc.contributor.authorEscosteguy-Neto, João Carlos [UNIFESP]
dc.contributor.authorCavarsan, Clarissa F. [UNIFESP]
dc.contributor.authorMello, Luiz E. [UNIFESP]
dc.contributor.authorSantos-Junior, Jair G. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFac Ciencias Med São Paulo
dc.description.abstractNeurogenesis in the subgranular layer of the dentate gyrus (DG) has been suggested to underlie some forms of associative learning. the present study was undertaken to determine whether there was also a role of neurogenesis in the ethanol (EtOH)-induced conditioned place preference (CPP). Outbreed Swiss mice were conditioned with EtOH (2.0g/kg) in one compartment of a non-biased place preference chamber and saline in the other compartment. This procedure produced three groups of mice: some developed a conditioned preference (EtOH_Cpp), others developed a conditioned avoidance (EtOH_Cpa) and still others demonstrated indifference to the context previously paired with ethanol (EtOH_Ind). BrdU (40mg/kg, i.p.) was administered 4 hours after each session comprising the conditioning phase. When measured 24 hours following the CPP test, there was no effect of EtOH on doublecortin (DCX) expression or Fluoro Jade B staining. However, there were decreases in the number of BrdU+ and Ki-67+ cells in the EtOH_Cpa and EtOH_Ind groups, but not in the EtOH_Cpp group. Most of BrdU+ cells were co-labeled with DCX. Similarly, in another experiment, in that the perfusion was done 28 days after CPP test, most BrdU+ cells were co-localized with NeuN. These results suggest that conditioned appetitive response is able to maintain normal levels of neurogenesis in DG and might counteract ethanol-produced decreased cell proliferation/survival rate.en
dc.description.affiliationUniversidade Federal de São Paulo, Neurobiol Lab, Grp Neuronal Plast & Psychiat Disorders, São Paulo, Brazil
dc.description.affiliationFac Ciencias Med São Paulo, Dept Ciencias Fisiol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Neurobiol Lab, Grp Neuronal Plast & Psychiat Disorders, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.identifier.citationAddiction Biology. Hoboken: Wiley-Blackwell, v. 18, n. 5, p. 774-785, 2013.
dc.relation.ispartofAddiction Biology
dc.rightsAcesso restrito
dc.subjectcell proliferationen
dc.subjectconditioned place preferenceen
dc.subjectneuronal degenerationen
dc.titleEffects of ethanol on hippocampal neurogenesis depend on the conditioned appetitive responseen