Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptides

dc.contributor.authorNakaie, Clovis Ryuichi [UNIFESP]
dc.contributor.authorBarbosa, Simone Reis [UNIFESP]
dc.contributor.authorVieira, Renata de Freitas Fischer [UNIFESP]
dc.contributor.authorFernandez, Roberto Morato
dc.contributor.authorCilli, Eduardo Maffud [UNIFESP]
dc.contributor.authorCastrucci, Ana Maria de Lauro
dc.contributor.authorVisconti, Maria Aparecida
dc.contributor.authorIto, Amando Siuiti
dc.contributor.authorLamy, Maria Teresa
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T12:31:23Z
dc.date.available2016-01-24T12:31:23Z
dc.date.issued2001-05-25
dc.description.abstractSimilar to melanocyte stimulating hormone (alpha -MSH), its potent and long-acting analogue, [Nle(4), D-Phe(7)]alpha -MSH, when labeled with the paramagnetic amino acid probe 2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acid (Toac), maintains its full biological potency, thus validating any comparative structural investigations between the two labeled peptides, Correlation times, calculated from tire electron paramagnetic resonance signal of Toac bound to the peptides, and Toac-Trp distances, estimated from the Toac fluorescence quenching of the Trp residue present in the peptides, indicate a more rigid and folded structure for the potent analogue as compared to the hormone, in aqueous medium. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Fis, BR-66318 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Biociencias, Dept Fisiol, BR-11176 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent103-107
dc.identifierhttps://dx.doi.org/10.1016/S0014-5793(01)02449-8
dc.identifier.citationFebs Letters. Amsterdam: Elsevier B.V., v. 497, n. 2-3, p. 103-107, 2001.
dc.identifier.doi10.1016/S0014-5793(01)02449-8
dc.identifier.fileWOS000169120900009.pdf
dc.identifier.issn0014-5793
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/26557
dc.identifier.wosWOS:000169120900009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFebs Letters
dc.rightsAcesso aberto
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectPeptideen
dc.subjectAlpha-melanocyte stimulating hormoneen
dc.subjectElectron paramagnetic resonanceen
dc.subject2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic aciden
dc.subjectFluorescenceen
dc.subjectSpin labelen
dc.titleComparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptidesen
dc.typeArtigo
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