Involvement of Ssp-4-related carbohydrate epitopes in mammalian cell invasion by Trypanosoma cruzi amastigotes

Página do item simplificado
dc.contributor.authorSilva, Claudio Vieira da
dc.contributor.authorLuquetti, Alejandro O.
dc.contributor.authorRassi, Anis
dc.contributor.authorMortara, Renato Arruda
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal de Goiás (UFG)
dc.date.accessioned2016-01-24T12:41:18Z
dc.date.available2016-01-24T12:41:18Z
dc.date.issued2006-07-01
dc.description.abstractWe examined whether the expression of Ssp-4-related carbohydrate epitopes defined by monoclonal antibodies ID9 and 2B7 was related to cell invasion by Trypanosonia cruzi amastigotes from different isolates and whether the highest expression of the epitope defined by MAb ID9 would confer greater infectivity. Confocal microscopy showed that both epitopes localize to the membrane of amastigotes from 569, 588, 573, 587 and SC2005 isolates, similar to the G isolate, whereas the CL isolate showed a punctate and diffuse staining. Flow cytometry revealed inter and intra-isolate variable expression of these epitopes. Apart from the lower expression of MAb 2B7 epitope by intracellular amastigotes of the SC2005 isolate, amastigotes from chagasic patient isolates expressed both epitopes similar to the G isolate, in contrast to CL isolate, that showed lower expression of both epitopes. MAb ID9 did not react with CL isolate on immunoblots and reacted poorly with 588 and 587 parasites. MAb 2B7 preferentially reacted with an epitope on an 84 kDa component in G and 573 isolates. Invasion assays revealed that despite the fact that arnastigotes from chagasic patient isolates displayed high levels of the epitope defined by MAb 1D9, only isolate 588 invaded host cells in levels comparable to that of isolate G. Both MAbs specifically inhibited cell invasion by G and 588, but not CL. These results suggested that the highest expression of MAb 1D9 epitope was not sufficient to confer higher infectivity on the isolate, and besides the two epitopes, other factors may modulate the invasiveness of extracellular amastigotes from the different isolates. (c) 2006 Elsevier SAS. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Fed Goias, Fac Med, Hosp Clin, Lab Doenca Chagas, Goiania, Go, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent2120-2129
dc.identifierhttp://dx.doi.org/10.1016/j.micinf.2006.03.016
dc.identifier.citationMicrobes and Infection. Amsterdam: Elsevier B.V., v. 8, n. 8, p. 2120-2129, 2006.
dc.identifier.doi10.1016/j.micinf.2006.03.016
dc.identifier.issn1286-4579
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29009
dc.identifier.wosWOS:000241115100018
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMicrobes and Infection
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectTrypanosoma cruzien
dc.subjectamastigoteen
dc.subjectcarbohydrate epitopesen
dc.subjectpolymorphismen
dc.subjectcell invasionen
dc.titleInvolvement of Ssp-4-related carbohydrate epitopes in mammalian cell invasion by Trypanosoma cruzi amastigotesen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
Em verificação - Geral