TGF-β1 on induced osteogenic differentiation of human dermal fibroblast

dc.contributor.authorAloise, Antonio Carlos [UNIFESP]
dc.contributor.authorPereira, Max Domingues
dc.contributor.authorDuailibi, Silvio Eduardo [UNIFESP]
dc.contributor.authorGragnani, Alfredo [UNIFESP]
dc.contributor.authorFerreira, Lydia Masako [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:46:48Z
dc.date.available2015-06-14T13:46:48Z
dc.date.issued2014-01-01
dc.description.abstractPURPOSE:To evaluate the role of transforming growth factor beta 1 (TGF-β1) on the induced osteogenic differentiation of human dermal fibroblasts.METHODS:We performed four groups with cultured dermal fibroblasts according to the culture medium: CONTROL (DMEM culture medium); TGF-β1 (DMEM culture medium with 10 ng/ml of TGF-β1); OSTEOG (DMEM culture medium with 0.5 µg/ml of ascorbic acid, 10 mmol/l of β-glycerophosphate and 10 nmol/L of dexamethasone); and OSTEOG/TGF-β1 (osteogenic medium with 10 ng/ml of TGF-β1). Alkaline phosphatase (ALP) activity and the amount of osteocalcin (OC) in the supernatant, as well as the capability to form calcium phosphate deposits, were analysed for 28 dayRESULTS:There were significant differences (p<0.05) between CONTROL and TGF-β1 groups in comparison with OSTEOG and OSTEOG/TGF-β1 groups in the ALP activity and OC amount. Although, both osteogenic groups had the same behavior with regard the expression curve during the experimental time, the OSTEOG/TGF-β1 group achieved significantly higher ALP and OC levels and showed no significant difference in the levels of mineralized deposits and in comparison with the levels found in the OSTEOG group.CONCLUSION:The addition of transforming growth factor beta 1 to the osteogenic culture medium increased the activity of alkaline phosphatase and the amount of osteocalcin, but TGF-β1 did not alter the presence of mineralized calcium phosphate deposits.en
dc.description.affiliationFederal University of São Paulo Department of Surgery
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Department of Surgery
dc.description.affiliationUnifespUNIFESP, Department of Surgery
dc.description.affiliationUnifespUNIFESP, Department of Surgery
dc.description.sourceSciELO
dc.format.extent01-06
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502014001300001
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 01-06, 2014.
dc.identifier.doi10.1590/S0102-86502014001300001
dc.identifier.fileS0102-86502014001300002.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502014001300002
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8124
dc.identifier.wosWOS:000342065000002
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsAcesso aberto
dc.subjectFibroblastsen
dc.subjectTransforming Growth Factor beta1en
dc.subjectDexamethasoneen
dc.subjectCell differentiationen
dc.titleTGF-β1 on induced osteogenic differentiation of human dermal fibroblasten
dc.typeArtigo
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