Decreased density of binding sites for the Ca2+ channel antagonist [3H] isradipine after denervation of rat vas deferens

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dc.contributor.authorJurkiewicz, Aron [UNIFESP]
dc.contributor.authorLafayette, Simone Sette Lopes [UNIFESP]
dc.contributor.authorNunes, S. H.
dc.contributor.authorMartini, L. C.
dc.contributor.authorDocarmo, LGD
dc.contributor.authorWanderley, A. G.
dc.contributor.authorJurkiewicz, N. H.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T11:40:18Z
dc.date.available2016-01-24T11:40:18Z
dc.date.issued1994-05-02
dc.description.abstractRadioligand binding assays were performed with the selective antagonist of dihydropyridine-sensitive Ca2+ channels [H-3]PN200-110 (isradipine) in rat vas deferens, before and 7 days after denervation, and data were compared with those obtained for K+-induced contractions, which are Ca2+-dependent. the density (B-max) of dihydropyridine binding sites was decreased to almost one-third of its normal value after denervation. the respective affinity (K-D) was not significantly changed. in addition, it was observed that the K+-induced tonic contraction, which corresponded to 55 +/- 2% of the respective phasic contraction, was decreased to 41 +/- 3% after: denervation. It is assumed that the decreased density of Ca2+ channels causes a decrease in K+-induced influx of Ca2+ and consequently of the corresponding tonic contraction. These results indicate that autonomic innervation can regulate the density of dihydropyridine-sensitive Ca2+ channels in the rat vas deferens.en
dc.description.affiliationESCOLA PAULISTA MED,DEPT PHARMACOL,CAIXA POSTAL 20372,BR-04034970 São Paulo,BRAZIL
dc.description.affiliationUnifespESCOLA PAULISTA MED,DEPT PHARMACOL,CAIXA POSTAL 20372,BR-04034970 São Paulo,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent329-333
dc.identifierhttps://dx.doi.org/10.1016/0014-2999(94)90559-2
dc.identifier.citationEuropean Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 256, n. 3, p. 329-333, 1994.
dc.identifier.doi10.1016/0014-2999(94)90559-2
dc.identifier.issn0014-2999
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/25407
dc.identifier.wosWOS:A1994NK58000012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofEuropean Journal of Pharmacology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectVAS DEFERENSen
dc.subjectDENERVATIONen
dc.subjectCA2+ CHANNELen
dc.subjectDIHYDROPYRIDINEen
dc.subjectISRADIPINEen
dc.titleDecreased density of binding sites for the Ca2+ channel antagonist [3H] isradipine after denervation of rat vas deferensen
dc.typeinfo:eu-repo/semantics/article
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