A recombinant vaccine based on domain II of Plasmodium vivax Apical Membrane Antigen 1 induces high antibody titres in mice

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dc.contributor.authorGentil, Fernanda
dc.contributor.authorBargieri, Daniel Y. [UNIFESP]
dc.contributor.authorLeite, Juliana A.
dc.contributor.authorFrancoso, Katia S.
dc.contributor.authorPatricio, Mariana B. M.
dc.contributor.authorEspindola, Noeli M.
dc.contributor.authorVaz, Adelaide J.
dc.contributor.authorPalatnik-de-Sousa, Clarisa B.
dc.contributor.authorRodrigues, Mauricio M. [UNIFESP]
dc.contributor.authorCosta, Fabio Trindade Maranhão [UNIFESP]
dc.contributor.authorSoares, Irene S.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.date.accessioned2016-01-24T14:05:20Z
dc.date.available2016-01-24T14:05:20Z
dc.date.issued2010-08-31
dc.description.abstractThe Apical Membrane Antigen 1 (AMA-1) is considered a promising candidate for development of a malaria vaccine against asexual stages of Plasmodium. We recently identified domain II (DII) of Plasmodium vivax AMA-1 (PvAMA-1) as a highly immunogenic region recognised by IgG antibodies present in many individuals during patent infection with P. vivax. the present study was designed to evaluate the immunogenic properties of a bacterial recombinant protein containing PvAMA-1 DII. To accomplish this, the recombinant protein was administered to mice in the presence of each of the following six adjuvants: Complete/Incomplete Freund's Adjuvant (CFA/IFA), aluminium hydroxide (Alum), Quil A, QS21 saponin, CpG-ODN 1826 and TiterMax. We found that recombinant DII was highly immunogenic in BALB/c mice when administered in the presence of any of the tested adjuvants. Importantly, we show that DII-specific antibodies recognised the native AMA-1 protein expressed on the surface of P. vivax merozoites isolated from the blood of infected patients. These results demonstrate that a recombinant protein containing PvAMA-1 DII is immunogenic when administered in different adjuvant formulations, and indicate that this region of the AMA-1 protein should continue to be evaluated as part of a subunit vaccine against vivax malaria. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, CINTERGEN, BR-04044010 São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Genet Evolucao & Bioagentes, Inst Biol, BR-13083862 Campinas, SP, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Goes, BR-21941902 Rio de Janeiro, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, CINTERGEN, BR-04044010 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipThe National Institute for Vaccine Development and Technology
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2006/02832-0
dc.format.extent6183-6190
dc.identifierhttp://dx.doi.org/10.1016/j.vaccine.2010.07.017
dc.identifier.citationVaccine. Oxford: Elsevier B.V., v. 28, n. 38, p. 6183-6190, 2010.
dc.identifier.doi10.1016/j.vaccine.2010.07.017
dc.identifier.issn0264-410X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32830
dc.identifier.wosWOS:000282150700011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofVaccine
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectMalariaen
dc.subjectPlasmodium vivaxen
dc.subjectRecombinant vaccineen
dc.titleA recombinant vaccine based on domain II of Plasmodium vivax Apical Membrane Antigen 1 induces high antibody titres in miceen
dc.typeinfo:eu-repo/semantics/article
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