Induction of interleukin-10 by HIV antigens in peripheral mononuclear cells of health care workers after occupational exposure to HIV-1-positive blood

dc.contributor.authorRodrigues, Denise do Socorro da Silva [UNIFESP]
dc.contributor.authorBrunialti, Milena Karina Coló [UNIFESP]
dc.contributor.authorMedeiros, Eduardo Alexandrino Servolo de [UNIFESP]
dc.contributor.authorDiaz, Ricardo Sobhie [UNIFESP]
dc.contributor.authorTurcato Junior, Gilberto [UNIFESP]
dc.contributor.authorSalomão, Reinaldo [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:29:43Z
dc.date.available2015-06-14T13:29:43Z
dc.date.issued2002-06-01
dc.description.abstractEvaluation of HIV-induced IL-2 production by peripheral blood mononuclear cells (PBMC) and HIV-specific T helper and cytotoxic T lymphocyte (CTL) responses in health care workers (HCW) occupationally exposed to HIV reveals a high rate of response to HIV among non-seroconverters. IL-10 is also known to interfere with HIV infection in vitro. To evaluate the induction of IL-10 by HIV antigens in HCW occupationally exposed to HIV, 18 HCW with percutaneous injury were enrolled in this study, 9 of them exposed to HIV-contaminated blood, and 9 exposed to HIV-negative blood. PBMC were incubated on plates coated with HIV-1 antigens, and IL-10 was measured in supernatants by ELISA. Five of nine HCW exposed to HIV-contaminated blood presented HIV-induced IL-10. Two of nine HCW exposed to HIV-negative source patients also had detectable levels of HIV-induced IL-10, one of them in the sample obtained on the day of accidental exposure. There was a relationship between the type of device involved in injury and IL-10 production. Individuals exposed to hollow needles or scalpels presented HIV-induced IL-10, whereas those exposed to solid needles and to digital puncture did not, suggesting a relationship between infectious load and IL-10. Although occupational exposure to HIV leads to a low rate of seroconversion, these individuals can develop an antigen-specific immune response characterized in our study by induction of IL-10 in PBMC in vitro.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Disciplina de Doenças Infecciosas e Parasitárias
dc.description.affiliationUnifespUNIFESP, EPM, Disciplina de Doenças Infecciosas e Parasitárias
dc.description.sourceSciELO
dc.format.extent697-701
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2002000600010
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 6, p. 697-701, 2002.
dc.identifier.doi10.1590/S0100-879X2002000600010
dc.identifier.fileS0100-879X2002000600010.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2002000600010
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/1459
dc.identifier.wosWOS:000176452400010
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsAcesso aberto
dc.subjectHIV-1en
dc.subjectInterleukin-10en
dc.subjectNeedlestick injuryen
dc.subjectOccupational exposureen
dc.subjectHealth care workeren
dc.titleInduction of interleukin-10 by HIV antigens in peripheral mononuclear cells of health care workers after occupational exposure to HIV-1-positive blooden
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