Antimicrobial susceptibility of photodynamic therapy (UVA/riboflavin) against Staphylococcus aureus

dc.contributor.authorKashiwabuchi, Renata Tiemi [UNIFESP]
dc.contributor.authorKhan, Yasin
dc.contributor.authorCarvalho, Fabio Ramos de Souza [UNIFESP]
dc.contributor.authorHirai, Flávio Eduardo [UNIFESP]
dc.contributor.authorCampos, Mauro Silveira de Queiroz [UNIFESP]
dc.contributor.authorMcDonnell, Peter John
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionJohns Hopkins Univ
dc.description.abstractPurpose: To assess S. aureus in vitro viability after the exposure to ultraviolet light A (UVA) and riboflavin (B2).Methods: Samples of S. aureus in 96 well plates (in triplicate) were exposed to riboflavin (B2) and ultraviolet light A (365 nm wavelength) at a power density of 3 mW/cm(2), 8 mm spot diameter, for 30 minutes. Control groups were prepared as well in triplicate: blank control, ultraviolet light A only, riboflavin only and dead bacteria Control. The bacterial viability was measured using fluorescent microscopy. In order to investigate the occurrence of viable but non-culturable microorganisms after treatment, the cell viability was also investigated by plate culture procedure onto a broth medium. Statistical analysis was performed using the triplicate values from each experimental condition.Results: No difference was observed among the treatment group and the control samples (p=1).Conclusion: The combination of riboflavin 0.1% and ultraviolet light A at 365 nm did not exhibit antimicrobial activity against oxacillin susceptible S. aureus.en
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Ophthalmol, Sao Paulo, Brazil
dc.description.affiliationJohns Hopkins Univ, Sch Med, Wilmer Ophthalmol Inst, Baltimore, MD 21205 USA
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Ophthalmol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipResearch to Prevent Blindness Inc, NY, NY
dc.identifier.citationArquivos Brasileiros De Oftalmologia. Sao Paulo: Consel Brasil Oftalmologia, v. 75, n. 6, p. 423-426, 2012.
dc.publisherConsel Brasil Oftalmologia
dc.relation.ispartofArquivos Brasileiros De Oftalmologia
dc.rightsAcesso aberto
dc.subjectCorneal ulceren
dc.subjectStaphylococcus aureusen
dc.subjectUltraviolet raysen
dc.subjectCross-linking reagentsen
dc.titleAntimicrobial susceptibility of photodynamic therapy (UVA/riboflavin) against Staphylococcus aureusen
dc.title.alternativeSuscetibilidade antimicrobiana da terapia fotodinâmica (UVA/riboflavina) contra Staphylococcus aureuspt