Evaluation of the genetic diversity of Histoplasma capsulatum var. capsulatum isolates from north-eastern Brazil

dc.contributor.authorBrilhante, Raimunda S. N.
dc.contributor.authorRibeiro, Joyce F.
dc.contributor.authorLima, Rita A. C.
dc.contributor.authorCastelo-Branco, Debora S. C. M.
dc.contributor.authorSoares, Rodrigo Martins
dc.contributor.authorMesquita, Jaco R. L.
dc.contributor.authorGrangeiro, Thalles Barbosa
dc.contributor.authorCamargo, Zoilo P. de [UNIFESP]
dc.contributor.authorCordeiro, Rossana A.
dc.contributor.authorRocha, Marcos F. G.
dc.contributor.authorSidrim, Jose J. C.
dc.contributor.institutionUniv Fed Ceara
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionSao Jose Hosp
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Estadual Ceara
dc.description.abstractSince the beginning of the HIV epidemic, there has been a significant increase in the number of histoplasmosis cases in Ceara, a state in north-east Brazil. the lack of epidemiological data on the genotypes circulating in the north-east region shows the importance of more detailed studies on the molecular epidemiology of Histoplasma capsulatum var. capsulatum in this region. Different molecular techniques have been used to better characterize the genetic profile of H. capsulatum var. capsulatum strains. the aim of this study was to analyse the genetic diversity of H. capsulatum var. capsulatum isolates in Fortaleza, the capital of Ceara, through the sequencing of the internal transcribed spacer (ITS)1-5.8S-ITS2 region, and establish the molecular profile of these isolates, along with strains from south-east Brazil, by RAPD analysis, featuring the different clusters in those regions. the isolates were grouped into two clusters. Cluster 1 included strains from the south-east and north-east regions with separation of isolates into three distinct subgroups (subgroups 1a, 1 b and 1 c). Cluster 2 included only samples from north-east Brazil. Sequencing of the ITS1 -5.8S-ITS2 region allowed the detection of two major clades, which showed geographical correlation between them and their subgroups. Therefore, it can be concluded that the H. capsulatum var. capsulatum isolates from Ceara have a high degree of genetic polymorphism. the molecular data also confirm that populations of this fungus are composed of different genotypes in Brazil and worldwide.en
dc.description.affiliationUniv Fed Ceara, Specialized Med Mycol Ctr, Postgrad Program Med Microbiol, Coll Med,Dept Pathol & Legal Med, Fortaleza, Ceara, Brazil
dc.description.affiliationUniv São Paulo, Coll Vet Med, Dept Prevent Vet Med & Anim Hlth, São Paulo, Brazil
dc.description.affiliationSao Jose Hosp, Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Fed Ceara, Dept Biol Sci, Fortaleza, Ceara, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUniv Estadual Ceara, Coll Vet Med, Postgrad Program Vet Sci, Fortaleza, Ceara, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDCNPq: 562296/2010-7
dc.description.sponsorshipIDCNPq: 552161/2011-0
dc.description.sponsorshipIDCNPq: 304779/2011-3
dc.description.sponsorshipIDCNPq: 473025/2012-4
dc.description.sponsorshipIDCAPES: 2103/2009
dc.identifier.citationJournal of Medical Microbiology. Reading: Soc General Microbiology, v. 61, n. 12, p. 1688-1695, 2012.
dc.publisherSoc General Microbiology
dc.relation.ispartofJournal of Medical Microbiology
dc.rightsAcesso aberto
dc.titleEvaluation of the genetic diversity of Histoplasma capsulatum var. capsulatum isolates from north-eastern Brazilen