CIRCULATING IMMUNE-COMPLEXES IN LEPROSY SERA - DEMONSTRATION OF ANTIBODIES AGAINST MYCOBACTERIAL GLYCOLIPIDIC ANTIGENS IN ISOLATED IMMUNE-COMPLEXES

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dc.contributor.authorTomimori, Jane [UNIFESP]
dc.contributor.authorCruaud, P.
dc.contributor.authorPapa, F.
dc.contributor.authorRotta, Osmar [UNIFESP]
dc.contributor.authorDavid, H. L.
dc.contributor.institutionINST PASTEUR
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionHOP JEAN VERDIER
dc.date.accessioned2018-06-15T17:44:22Z
dc.date.available2018-06-15T17:44:22Z
dc.date.issued1993-03-01
dc.description.abstractCirculating immune complexes (CIC) were assayed in sera of leprosy patients. Using an immunoassay for two mycobacterial antigens-phenolic glycolipid-I (PGL-I) and glycolipid IV (SL-IV)-sera from 65 patients with leprosy (38 lepromatous, 18 borderline, and 9 tuberculoid) were studied. The CIC were isolated by polyethylene glycol (PEG) precipitation, washed, treated with an acid buffer, neutralized, and tested using an enzyme-linked immunosorbent assay (ELISA). We demonstrated that CIC could contain IgG and IgM antibodies reacting against PGL-I and SL-IV. The high levels of antibodies in the precipitable CIC showed concordance with high levels in the original sera, although some patients presented high levels of precipitable CIC in the absence of high titers of antibodies in their sera. It was concluded that some of the CIC observed in patients with leprosy were composed of IgG and IgM immunoglobulins against specific mycobacterial antigens.en
dc.description.affiliationINST PASTEUR,UNITE TB & MYCOBACTERIES,F-75724 PARIS 15,FRANCE
dc.description.affiliationESCOLA PAULISTA MED,DEPT DERMATOL,BR-04023 SAO PAULO,BRAZIL
dc.description.affiliationHOP JEAN VERDIER,F-93140 BONDY,FRANCE
dc.description.affiliationUnifespESCOLA PAULISTA MED,DEPT DERMATOL,BR-04023 SAO PAULO,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent44-50
dc.identifierhttp://www.leprosy-ila.org/leprosyjournal/gn1/detalhe_artigo.php?id=MTA2Mw%3D%3D&secao=ORIGINAL+ARTICLE
dc.identifier.citationInternational Journal Of Leprosy And Other Mycobacterial Diseases. Baton Rouge: Amer Leprosy Mission, v. 61, n. 1, p. 44-50, 1993.
dc.identifier.issn0148-916X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/44051
dc.identifier.wosWOS:A1993LN70100008
dc.language.isoeng
dc.publisherAmer Leprosy Mission
dc.relation.ispartofInternational Journal Of Leprosy And Other Mycobacterial Diseases
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleCIRCULATING IMMUNE-COMPLEXES IN LEPROSY SERA - DEMONSTRATION OF ANTIBODIES AGAINST MYCOBACTERIAL GLYCOLIPIDIC ANTIGENS IN ISOLATED IMMUNE-COMPLEXESen
dc.typeinfo:eu-repo/semantics/article
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