Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli

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dc.citation.issue4
dc.citation.volume84
dc.contributor.authorSampaio, Suely Carlos Ferreira [UNIFESP]
dc.contributor.authorLuiz, Wilson B.
dc.contributor.authorVieira, Monica Aparecida Midolli [UNIFESP]
dc.contributor.authorFerreira, Rita C. C.
dc.contributor.authorGarcia, Bruna Gil [UNIFESP]
dc.contributor.authorSinigaglia-Coimbra, Rita [UNIFESP]
dc.contributor.authorSampaio, Jorge L. M.
dc.contributor.authorFerreira, Luis C. S.
dc.contributor.authorGomes, Tania Aparecida Tardelli [UNIFESP]
dc.coverageWashington
dc.date.accessioned2020-07-22T13:23:12Z
dc.date.available2020-07-22T13:23:12Z
dc.date.issued2016
dc.description.abstractThe expression of flagella correlates with different aspects of bacterial pathogenicity, ranging from adherence to host cells to activation of inflammatory responses by the innate immune system. In the present study, we investigated the role of flagella in the adherence of an atypical enteropathogenic Escherichia coli (aEPEC) strain (serotype O51: H40) to human enterocytes. Accordingly, isogenic mutants deficient in flagellin (FliC), the flagellar structural subuniten
dc.description.abstractthe flagellar cap protein (FliD)en
dc.description.abstractor the MotAB proteins, involved in the control of flagellar motion, were generated and tested for binding to differentiated Caco-2 cells. Binding of the aEPEC strain to enterocytes was significantly impaired in strains with the fliC and fliD genes deleted, both of which could not form flagella on the bacterial surface. A nonmotile but flagellated MotAB mutant also showed impaired adhesion to Caco-2 cells. In accordance with these observations, adhesion of aEPEC strain 1711-4 to Caco-2 cells was drastically reduced after the treatment of Caco-2 cells with purified FliD. In addition, incubation of aEPEC bacteria with specific anti-FliD serum impaired binding to Caco-2 cells. Finally, incubation of Caco-2 cells with purified FliD, followed by immunolabeling, showed that the protein was specifically bound to the microvillus tips of differentiated Caco-2 cells. The aEPEC FliD or anti-FliD serum also reduced the adherence of prototype typical enteropathogenic, enterohemorrhagic, and enterotoxigenic E. coli strains to Caco-2 cells. In conclusion, our findings further strengthened the role of flagella in the adherence of aEPEC to human enterocytes and disclosed the relevant structural and functional involvement of FliD in the adhesion process.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Ctr Microscopia Eletron, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Ciencias Farmaceut, Sao Paulo, Brazil
dc.description.affiliationInst Fleury Ensino & Pesquisa, Sao Paulo, Brazil
dc.description.affiliationFac Ciencias Med Santa Casa Sao Paulo, Dept Patol, Div Microbiol, Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Santa Cruz, Ctr Biotecnol & Genet, Ilheus, Bahia, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Ctr Microscopia Eletron, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipIDFAPESP: 2011/12664-5
dc.description.sponsorshipIDFAPESP: 2009/50399-1
dc.description.sponsorshipIDCNPq: 150833/2012-1
dc.format.extent1112-1122
dc.identifierhttp://dx.doi.org/10.1128/IAI.01001-15
dc.identifier.citationInfection And Immunity. Washington, v. 84, n. 4, p. 1112-1122, 2016.
dc.identifier.doi10.1128/IAI.01001-15
dc.identifier.issn0019-9567
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56093
dc.identifier.wosWOS:000377103600023
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.relation.ispartofInfection And Immunity
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleFlagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvillien
dc.typeinfo:eu-repo/semantics/article
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