Comparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcome

dc.contributor.authorSantos, Ingrid Nayara Marcelino [UNIFESP]
dc.contributor.authorKurihara, Mariana Neri Lucas [UNIFESP]
dc.contributor.authorSantos, Fernanda Fernandes
dc.contributor.authorValiatti, Tiago Barcelos [UNIFESP]
dc.contributor.authorSilva, Juliana Thalita Paulino [UNIFESP]
dc.contributor.authorPignatari, Antonio Carlos Campos [UNIFESP]
dc.contributor.authorSalles, Mauro Jose Costa [UNIFESP]
dc.contributor.authorLatteshttp://lattes.cnpq.br/7196518766323325pt_BR
dc.contributor.authorLatteshttp://lattes.cnpq.br/0214364156717092
dc.contributor.authorLatteshttp://lattes.cnpq.br/6922450552975380
dc.contributor.authorLatteshttp://lattes.cnpq.br/1570873280199853
dc.contributor.authorLatteshttp://lattes.cnpq.br/9461346610553865
dc.contributor.authorLatteshttp://lattes.cnpq.br/3119933864520484
dc.date.accessioned2022-06-09T17:44:39Z
dc.date.available2022-06-09T17:44:39Z
dc.date.issued2022-06-02
dc.description.abstractStaphylococcus spp. remain the leading biofilm-forming agents causing orthopedic im-plant-associated infections (OIAI). This is a descriptive study of phenotypic and genomic features identified in clinical isolates of S. aureus and coagulase-negative Staphylococcus (CoNS) recovered from OIAIs patients that progressed to treatment failure. Ten isolates were identified by matrix-time-of-flight laser-assisted desorption mass spectrometry (MALDI-TOF-MS) and tested for antibi-otic susceptibility and biofilm formation. Genotypic characteristics, including, MLST (Multi Locus Sequence Typing), SCCmec typing, virulence and resistance genes were assessed by whole-genome sequencing (WGS). All S. aureus harbored mecA, blaZ, and multiple resistance genes for aminogly-cosides and quinolones. All MRSA were strong biofilm producers harboring the complete icaADBC and icaR operon. Seven CoNS isolates comprising five species (S. epidermidis, S. haemolyticus, S. sci-uri, S. capitis and S. lugdunensis) were analyzed, with mecA gene detected in five isolates. S. haemoli-tycus (isolate 95), and S. lugdunensis were unable to form biofilm and did not harbor the complete icaADBCR operon. High variability of adhesion genes was detected, with atl, ebp, icaADBC operon, and IS256 being the most common. In conclusion, MRSA and CoNS isolates carrying genes for bio-film production, and resistance to β-lactam and aminoglycosides are associated with treatment fail-ure in OIAIs.en
dc.identifierhttps://doi.org/10.3390/microorganisms10061149pt_BR
dc.identifier.doi10.3390/microorganisms10061149pt_BR
dc.identifier.urihttps://hdl.handle.net/11600/63955
dc.languageengpt_BR
dc.publisherMDPIpt_BR
dc.relation.ispartofMicroorganismsen
dc.rightsAcesso abertopt_BR
dc.subjectStaphylococcus aureusen
dc.subjectcoagulase-negative Staphylococcien
dc.subjectBiofilmen
dc.subjectOrthopedic infectionsen
dc.subjectSonicationen
dc.subjectbacterial resistancept_BR
dc.subjectwhole-genome sequencingpt_BR
dc.titleComparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcomeen
dc.typeArtigopt_BR
unifesp.campusEscola Paulista de Medicina (EPM)pt_BR
unifesp.departamentoMedicinapt_BR
unifesp.graduacaoBiomedicinapt_BR
unifesp.graduateProgramInfectologiapt_BR
unifesp.knowledgeAreaDoenças infecciosas e parasitáriaspt_BR
unifesp.researchAreaInfecções Associadas aos Biofilmespt_BR
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