Phylogenetic Analysis of Stenotrophomonas spp. Isolates Contributes to the Identification of Nosocomial and Community-Acquired Infections

dc.contributor.authorCerezer, Vinicius Godoy
dc.contributor.authorBando, Silvia Yumi
dc.contributor.authorPasternak, Jacyr [UNIFESP]
dc.contributor.authorFranzolin, Marcia Regina
dc.contributor.authorMoreira-Filho, Carlos Alberto
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionHosp Israelita Albert Einstein
dc.contributor.institutionInst Butantan
dc.description.abstractStenotrophomonas ssp. has a wide environmental distribution and is also found as an opportunistic pathogen, causing nosocomial or community-acquired infections. One species, S. maltophilia, presents multidrug resistance and has been associated with serious infections in pediatric and immunocompromised patients. Therefore, it is relevant to conduct resistance profile and phylogenetic studies in clinical isolates for identifying infection origins and isolates with augmented pathogenic potential. Here, multilocus sequence typing was performed for phylogenetic analysis of nosocomial isolates of Stenotrophomonas spp. and, environmental and clinical strains of S. maltophilia. Biochemical andmultidrug resistance profiles of nosocomial and clinical strains were determined. the inferred phylogenetic profile showed high clonal variability, what correlates with the adaptability process of Stenotrophomonas to different habitats. Two clinical isolates subgroups of S. maltophilia sharing high phylogenetic homogeneity presented intergroup recombination, thus indicating the high permittivity to horizontal gene transfer, a mechanism involved in the acquisition of antibiotic resistance and expression of virulence factors. for most of the clinical strains, phylogenetic inference was made using only partial ppsA gene sequence. Therefore, the sequencing of just one specific fragment of this gene would allow, in many cases, determining whether the infection with S. maltophilia was nosocomial or community-acquired.en
dc.description.affiliationUniv São Paulo, Fac Med, Dept Pediat, BR-05403900 São Paulo, Brazil
dc.description.affiliationHosp Israelita Albert Einstein, BR-05652900 São Paulo, Brazil
dc.description.affiliationInst Butantan, Bacteriol Lab, BR-05503900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2010/04115-9
dc.identifier.citationBiomed Research International. New York: Hindawi Publishing Corporation, 13 p., 2014.
dc.publisherHindawi Publishing Corporation
dc.relation.ispartofBiomed Research International
dc.rightsAcesso aberto
dc.titlePhylogenetic Analysis of Stenotrophomonas spp. Isolates Contributes to the Identification of Nosocomial and Community-Acquired Infectionsen
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