Effects of Cigarette Smoke and Ethanol Intake on Mouse Oesophageal Mucosa Changes Induced by Dietary Zinc Deficiency and Deoxycholic Acid Supplementation

dc.contributor.authorZapaterini, Joyce Regina
dc.contributor.authorMoura, Nelci Antunes de
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.authorRodrigues, Maria Aparecida Marchesan
dc.contributor.authorBarbisan, Luis Fernando
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:27:29Z
dc.date.available2016-01-24T14:27:29Z
dc.date.issued2012-08-01
dc.description.abstractThe noxious effects of dietary zinc deficiency (ZD) and deoxycholic bile acid (DCA) supplementation in the oesophagus were investigated. the additional influence of cigarette smoke and ethanol intake on the changes in the oesophageal mucosa induced by dietary ZD plus DCA was also assessed. Male C57BL/6 mice were allocated into four groups: Group 1 was fed control diet and groups 24 were fed ZD plus DCA diet. After 5 weeks, groups 3 and 4 were exposed to 10% ethanol intake or cigarette smoke for 15 weeks, respectively. All animals were euthanized at the end of week 20, and the oesophagus, lung, liver and colon were collected and analysed by conventional morphology. Cell proliferation was assessed in the oesophageal mucosa by Ki-67 immunohistochemistry and cyclooxygenase 2 (COX-2) protein by Western blotting. Dietary ZD plus DCA treatment induced mild hyperkeratosis and hyperplasia, increased cell proliferation index and COX-2 protein expression in the oesophagus, and intranuclear inclusion, karyocytomegaly and microvesicular fatty change in the liver. Cigarette smoke increased COX-2 protein expression in oesophageal mucosa and irregular enlargement of alveolus and alveolar ductal air spaces, while ethanol enhanced liver damage induced by ZD plus DCA diet. These findings indicate that dietary ZD plus DCA treatment during 20 weeks induces a pattern of chemical oesophageal injury but not Barrett's-like lesions.en
dc.description.affiliationSão Paulo State Univ, UNESP, Inst Biosci, Dept Morphol, Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Sch Med, Dept Pathol, Botucatu, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Biosci, Santos, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Biosci, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 08/53366-4
dc.description.sponsorshipIDFAPESP: 2009/50854-0
dc.description.sponsorshipIDFAPESP: 2007/57544-1
dc.format.extent92-98
dc.identifierhttp://dx.doi.org/10.1111/j.1742-7843.2012.00867.x
dc.identifier.citationBasic & Clinical Pharmacology & Toxicology. Hoboken: Wiley-Blackwell, v. 111, n. 2, p. 92-98, 2012.
dc.identifier.doi10.1111/j.1742-7843.2012.00867.x
dc.identifier.issn1742-7835
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35106
dc.identifier.wosWOS:000306401600005
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofBasic & Clinical Pharmacology & Toxicology
dc.rightsAcesso aberto
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.titleEffects of Cigarette Smoke and Ethanol Intake on Mouse Oesophageal Mucosa Changes Induced by Dietary Zinc Deficiency and Deoxycholic Acid Supplementationen
dc.typeArtigo
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