Interferon-alpha receptor 1 mRNA expression in peripheral blood mononuclear cells is associated with response to interferon-alpha therapy of patients with chronic hepatitis C

dc.contributor.authorMassirer, Katlin Brauer
dc.contributor.authorHirata, Mario Hiroyuki
dc.contributor.authorSilva, Antonio Eduardo Benedito [UNIFESP]
dc.contributor.authorFerraz, Maria Lucia Cardoso Gomes [UNIFESP]
dc.contributor.authorNguyen, Nga Yen
dc.contributor.authorHirata, Rosario Dominguez Crespo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFDA Center for Biological Evaluation and Research
dc.date.accessioned2015-06-14T13:30:22Z
dc.date.available2015-06-14T13:30:22Z
dc.date.issued2004-05-01
dc.description.abstractInterferon (IFN)-alpha receptor mRNA expression in liver of patients with chronic hepatitis C has been shown to be a response to IFN-alpha therapy. The objective of the present study was to determine whether the expression of mRNA for subunit 1 of the IFN-alpha receptor (IFNAR1) in peripheral blood mononuclear cells (PBMC) is associated with the response to IFN-alpha in patients with chronic hepatitis C. Thirty patients with positive anti-HCV and HCV-RNA, and abnormal levels of alanine aminotransferase in serum were selected and treated with IFN-alpha2b for one year. Those with HBV or HIV infection, or using alcohol were not included. Thirteen discontinued the treatment and were not evaluated. The IFN-alpha response was monitored on the basis of alanine aminotransferase level and positivity for HCV-RNA in serum. IFNAR1-mRNA expression in PBMC was measured by reverse transcription-polymerase chain reaction before and during the first three months of therapy. The results are reported as IFNAR1-mRNA/ß-actin-mRNA ratio (mean ± SD). Before treatment, responder patients had significantly higher IFNAR1-mRNA expression in PBMC (0.67 ± 0.15; N = 5; P < 0.05) compared to non-responders (0.35 ± 0.17; N = 12) and controls (0.30 ± 0.16; N = 9). Moreover, IFNAR1-mRNA levels were significantly reduced after 3 months of treatment in responders, whereas there were no differences in IFNAR1 expression in non-responders during IFN-alpha therapy. Basal IFNAR1-mRNA expression was not correlated with the serum level of alanine and aspartate aminotransferases or the presence of cirrhosis. The present results suggest that IFNAR1-mRNA expression in PBMC is associated with IFN-alpha response to hepatitis C and may be useful for monitoring therapy in patients with chronic hepatitis C.en
dc.description.affiliationUniversidade de São Paulo Faculdade de Ciências Farmacêuticas Departamento de Análises Clínicas e Toxicológicas
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Gastroenterologia
dc.description.affiliationFDA Center for Biological Evaluation and Research
dc.description.affiliationUnifespUNIFESP, Depto. de Gastroenterologia
dc.description.sourceSciELO
dc.format.extent643-647
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2004000500003
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 5, p. 643-647, 2004.
dc.identifier.doi10.1590/S0100-879X2004000500003
dc.identifier.fileS0100-879X2004000500003.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2004000500003
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/2082
dc.identifier.wosWOS:000221369800003
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsAcesso aberto
dc.subjectIFNAR1-mRNA expressionen
dc.subjectInterferon-alpha therapyen
dc.subjectChronic hepatitis Cen
dc.subjectInterferon-alpha receptoren
dc.subjectPeripheral blood mononuclear cellsen
dc.subjectReverse transcription- polymerase chain reactionen
dc.titleInterferon-alpha receptor 1 mRNA expression in peripheral blood mononuclear cells is associated with response to interferon-alpha therapy of patients with chronic hepatitis Cen
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