Identification of a new hepatitis B virus recombinant D2/D3 in the city of So Paulo, Brazil

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dc.citation.issue2
dc.citation.volume162
dc.contributor.authorSantana, Luiz Claudio [UNIFESP]
dc.contributor.authorMantovani, Nathalia Pena [UNIFESP]
dc.contributor.authorFerreira, Maira Cicero [UNIFESP]
dc.contributor.authorArnold, Rafael [UNIFESP]
dc.contributor.authorSanz Duro, Rodrigo Lopes [UNIFESP]
dc.contributor.authorAbrao Ferreira, Paulo Roberto [UNIFESP]
dc.contributor.authorHunter, James Richard [UNIFESP]
dc.contributor.authorLeal, Elcio
dc.contributor.authorDiaz, Ricardo Sobhie [UNIFESP]
dc.contributor.authorKomninakis, Shirley Vasconcelos [UNIFESP]
dc.coverageWien
dc.date.accessioned2020-07-17T14:03:07Z
dc.date.available2020-07-17T14:03:07Z
dc.date.issued2017
dc.description.abstractTwo hundred forty million people are chronically infected with hepatitis B virus (HBV) worldwide. The rise of globalization has facilitated the emergence of novel HBV recombinants and genotypes. We evaluated HBV genotypes and recombinants, mutations associated with resistance to antivirals (AVs), progression of hepatic illness, and inefficient hepatitis B vaccination responses in chronically infected individuals in the city of So Paulo, Brazil. Forty-five full-length and 24 partial-length sequences were obtained. The genotype distribution was as follows: A (66.7%), D (15.9%), F (11.6%) and C (4.3%). We describe a new recombinant (D2/D3), confirmed through next-generation sequencing (NGS) and reconstruction of the quasispecies sequences in silico. Primary resistance and major vaccine escape mutations were not found. We did, however, find mutations in the S region that might may be related to HBV antigenicity changes, as well as Pre-S deletions. The precore/core mutations A1762T + G1764A (40.9%) were found mostly in genotypes A and D, and G1896A (29.55%) was more frequent in genotype D than in genotype A. The genotypic distribution reflects the history of Brazilian immigration. This is the first description of recombination between genotypes D2 and D3 in Brazil. It is also the first confirmation through NGS and reconstruction of the quasispecies in silico. However, little is known about the response to treatment of recombinants. This demonstrates the need for molecular epidemiology studies involving the analysis of full-length HBV sequences.en
dc.description.affiliationUniv Fed Sao Paulo, Retrovirol Lab, 781 Pedro Toledo St,16 Floor, BR-04039032 Sao Paulo, SP, Brazil
dc.description.affiliationFed Univ Para, Inst Biol Sci, 01 Augusto Correa St, BR-66075110 Belem, Para, Brazil
dc.description.affiliationMed Sch ABC FMABC, Clin Immunol Lab, 821 Principe de Gales Av, BR-09060650 Santo Andre, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Retrovirol Lab, 781 Pedro Toledo St,16 Floor, BR-04039032 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP)
dc.description.sponsorshipNational Council of Technological and Scientific Development (CNPq)
dc.description.sponsorshipIDFAPESP: 2012/13285-0
dc.description.sponsorshipIDCNPq: 141858/2013-3
dc.format.extent457-467
dc.identifierhttp://dx.doi.org/10.1007/s00705-016-3122-2
dc.identifier.citationArchives Of Virology. Wien, v. 162, n. 2, p. 457-467, 2017.
dc.identifier.doi10.1007/s00705-016-3122-2
dc.identifier.issn0304-8608
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55178
dc.identifier.wosWOS:000394315600014
dc.language.isoeng
dc.publisherSpringer Wien
dc.relation.ispartofArchives Of Virology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.titleIdentification of a new hepatitis B virus recombinant D2/D3 in the city of So Paulo, Brazilen
dc.typeinfo:eu-repo/semantics/article
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