Genome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzi

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dc.contributor.authorSouza, Renata Torres [UNIFESP]
dc.contributor.authorLima, Fabio Mitsuo [UNIFESP]
dc.contributor.authorBarros, Roberto Moraes [UNIFESP]
dc.contributor.authorCortez, Danielle Rodrigues [UNIFESP]
dc.contributor.authorSantos, Michele Fernandes [UNIFESP]
dc.contributor.authorCordero, Esteban Mauricio [UNIFESP]
dc.contributor.authorRuiz, Jeronimo Conceição
dc.contributor.authorGoldenberg, Samuel
dc.contributor.authorTeixeira, Marta Maria Geraldes
dc.contributor.authorSilveira, José Franco da [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionCtr Pesquisas Rene Rachou Fiocruz
dc.contributor.institutionInst Carlos Chagas
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:17:05Z
dc.date.available2016-01-24T14:17:05Z
dc.date.issued2011-08-12
dc.description.abstractBackground: the Trypanosoma cruzi genome was sequenced from a hybrid strain (CL Brener). However, high allelic variation and the repetitive nature of the genome have prevented the complete linear sequence of chromosomes being determined. Determining the full complement of chromosomes and establishing syntenic groups will be important in defining the structure of T. cruzi chromosomes. A large amount of information is now available for T. cruzi and Trypanosoma brucei, providing the opportunity to compare and describe the overall patterns of chromosomal evolution in these parasites.Methodology/Principal Findings: the genome sizes, repetitive DNA contents, and the numbers and sizes of chromosomes of nine strains of T. cruzi from four lineages (TcI, TcII, TcV and TcVI) were determined. the genome of the TcI group was statistically smaller than other lineages, with the exception of the TcI isolate Tc1161 (Jose-IMT). Satellite DNA content was correlated with genome size for all isolates, but this was not accompanied by simultaneous amplification of retrotransposons. Regardless of chromosomal polymorphism, large syntenic groups are conserved among T. cruzi lineages. Duplicated chromosome-sized regions were identified and could be retained as paralogous loci, increasing the dosage of several genes. By comparing T. cruzi and T. brucei chromosomes, homologous chromosomal regions in T. brucei were identified. Chromosomes Tb9 and Tb11 of T. brucei share regions of syntenic homology with three and six T. cruzi chromosomal bands, respectively.Conclusions: Despite genome size variation and karyotype polymorphism, T. cruzi lineages exhibit conservation of chromosome structure. Several syntenic groups are conserved among all isolates analyzed in this study. the syntenic regions are larger than expected if rearrangements occur randomly, suggesting that they are conserved owing to positive selection. Mapping of the syntenic regions on T. cruzi chromosomal bands provides evidence for the occurrence of fusion and split events involving T. brucei and T. cruzi chromosomes.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationCtr Pesquisas Rene Rachou Fiocruz, Fiocruz, MG, Brazil
dc.description.affiliationInst Carlos Chagas, Curitiba, Parana, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent14
dc.identifierhttps://dx.doi.org/10.1371/journal.pone.0023042
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 6, n. 8, 14 p., 2011.
dc.identifier.doi10.1371/journal.pone.0023042
dc.identifier.fileWOS000293953500015.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/33954
dc.identifier.wosWOS:000293953500015
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleGenome Size, Karyotype Polymorphism and Chromosomal Evolution in Trypanosoma cruzien
dc.typeinfo:eu-repo/semantics/article
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