MUTATIONS IN THE QUINOLONE RESISTANCE-DETERMINING REGIONS OF GYRA AND PARC IN ENTEROBACTERIACEAE ISOLATES FROM BRAZIL

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dc.contributor.authorMinarini, Luciene Andrade da Rocha [UNIFESP]
dc.contributor.authorDarini, Ana Lucia C.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T16:24:03Z
dc.date.available2018-06-15T16:24:03Z
dc.date.issued2012-10-01
dc.description.abstractMutations in the quinolone resistance-determining regions (QRDR) in chromosomal gyrA and parC genes and fluoroquinolone susceptibility profiles were investigated in quinolone-resistant Enterobacteriaceae isolated from community and hospitalized patients in the Brazilian Southeast region. A total of 112 nalidixic acid-resistant enterobacterial isolates collected from 2000 to 2005 were investigated for mutations in the topoisomerases genes gyrA and parC by amplifying and sequencing the QRDR regions. Susceptibility to fluoroquinolones was tested by the agar dilution method. Amongst the 112 enterobacterial isolates, 81 (72.3%) were resistant to ciprofloxacin and 5 (4.5%) showed reduced susceptibility. Twenty-six (23.2%) were susceptible to ciprofloxacin. Several alterations were detected in gyrA and parC genes. Escherichia coli isolates (47.7%) showed double mutations in the gyrA gene and a single one in the parC gene. Two unusual aminoacid substitutions are reported, an Asp87-Asn in a Citrobacter freundii isolate with reduced susceptibility to fluoroquinolones and a Glu84-Ala in one E. coli isolate. Only a parC gene mutation was found in fluoroquinolone-susceptible Enterobacter aerogenes. None of the isolates susceptible to ciprofloxacin presented mutations in topoisomerase genes. This comprehensive analysis of QRDRs in gyrA and parC genes, covering commonly isolated Enterobacteriaceae in Brazil is the largest reported up to now.en
dc.description.affiliationUniv Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Diadema, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2006/02320-9
dc.format.extent1309-1314
dc.identifierhttp://dx.doi.org/10.1590/S1517-83822012000400010
dc.identifier.citationBrazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 43, n. 4, p. 1309-1314, 2012.
dc.identifier.doi10.1590/S1517-83822012000400010
dc.identifier.fileS1517-83822012000400010.pdf
dc.identifier.scieloS1517-83822012000400010
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43137
dc.identifier.wosWOS:000315286400010
dc.language.isoeng
dc.publisherSoc Brasileira Microbiologia
dc.relation.ispartofBrazilian Journal Of Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectquinolone resistance-determining regionen
dc.subjectmutationsen
dc.subjectgyrAen
dc.subjectparCen
dc.subjectEnterobacteriaceaeen
dc.titleMUTATIONS IN THE QUINOLONE RESISTANCE-DETERMINING REGIONS OF GYRA AND PARC IN ENTEROBACTERIACEAE ISOLATES FROM BRAZILen
dc.typeinfo:eu-repo/semantics/article
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