Lipidomic Analysis of Extracellular Vesicles from the Pathogenic Phase of Paracoccidioides brasiliensis

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dc.contributor.authorVallejo, Milene C. [UNIFESP]
dc.contributor.authorNakayasu, Ernesto S.
dc.contributor.authorLongo, Larissa V. G. [UNIFESP]
dc.contributor.authorGaniko, Luciane
dc.contributor.authorLopes, Felipe G.
dc.contributor.authorMatsuo, Alisson L. [UNIFESP]
dc.contributor.authorAlmeida, Igor C.
dc.contributor.authorPuccia, Rosana [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Texas El Paso
dc.date.accessioned2016-01-24T14:27:22Z
dc.date.available2016-01-24T14:27:22Z
dc.date.issued2012-06-22
dc.description.abstractBackground: Fungal extracellular vesicles are able to cross the cell wall and transport molecules that help in nutrient acquisition, cell defense, and modulation of the host defense machinery.Methodology/Principal Findings: Here we present a detailed lipidomic analysis of extracellular vesicles released by Paracoccidioides brasiliensis at the yeast pathogenic phase. We compared data of two representative isolates, Pb3 and Pb18, which have distinct virulence profiles and phylogenetic background. Vesicle lipids were fractionated into different classes and analyzed by either electrospray ionization- or gas chromatography-mass spectrometry. We found two species of monohexosylceramide and 33 phospholipid species, including phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid, phosphatidylserine, phosphatidylinositol, and phosphatidylglycerol. Among the phospholipid-bound fatty acids in extracellular vesicles, C181 predominated in Pb3, whereas C18:2 prevailed in Pb18. the prevalent sterol in Pb3 and Pb18 vesicles was brassicasterol, followed by ergosterol and lanosterol. Inter-isolate differences in sterol composition were observed, and also between extracellular vesicles and whole cells.Conclusions/Significance: the extensive lipidomic analysis of extracellular vesicles from two P. brasiliensis isolates will help to understand the composition of these fungal components/organelles and will hopefully be useful to study their biogenesis and role in host-pathogen interactions.en
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUniv Texas El Paso, Dept Biol Sci, Border Biomed Res Ctr, El Paso, TX 79968 USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipNational Institutes of Health (NIH)
dc.description.sponsorshipIDFAPESP: 06/05095-6
dc.description.sponsorshipIDFAPESP: 07/04757-8
dc.description.sponsorshipIDFAPESP: 07/59768-4
dc.description.sponsorshipIDCNPq: 301666/2010-5
dc.description.sponsorshipIDNational Institutes of Health (NIH): 5G12RR008124-16A1
dc.description.sponsorshipIDNational Institutes of Health (NIH): 5G12RR008124-16A1S1
dc.description.sponsorshipIDNational Institutes of Health (NIH): G12MD007592
dc.format.extent10
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0039463
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 7, n. 6, 10 p., 2012.
dc.identifier.doi10.1371/journal.pone.0039463
dc.identifier.fileWOS000305730900064.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35000
dc.identifier.wosWOS:000305730900064
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleLipidomic Analysis of Extracellular Vesicles from the Pathogenic Phase of Paracoccidioides brasiliensisen
dc.typeinfo:eu-repo/semantics/article
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