Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg

dc.contributor.authorMendes, Rita Gabriela Pedrosa Ribeiro
dc.contributor.authorGusmão, Michélia Antônia do Nascimento [UNIFESP]
dc.contributor.authorMaia, Ana Carolina Ribeiro Gomes
dc.contributor.authorDetoni, Michelle de Lima
dc.contributor.authorPorcino, Gabriane Nascimento
dc.contributor.authorSoares, Thais Vieira
dc.contributor.authorJuliano, Maria Aparecida [UNIFESP]
dc.contributor.authorJuliano, Luiz [UNIFESP]
dc.contributor.authorCoelho, Paulo Marcos Zech
dc.contributor.authorLenzi, Henrique Leonel
dc.contributor.authorFaria-Pinto, Priscila
dc.contributor.authorVasconcelos, Eveline Gomes
dc.contributor.institutionUniversidade Federal de Juiz de Fora Instituto de Ciências Biológicas Departamento de Bioquímica
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInstituto de Pesquisas René Rachou-Fiocruz
dc.contributor.institutionInstituto Oswaldo Cruz-Fiocruz Laboratório de Patologia
dc.date.accessioned2015-06-14T13:43:20Z
dc.date.available2015-06-14T13:43:20Z
dc.date.issued2011-11-01
dc.description.abstractA peptide (SmB2LJ; r175-194) that belongs to a conserved domain from Schistosoma mansoni SmATPDase 2 and is shared with potato apyrase, as predicted by in silico analysis as antigenic, was synthesised and its immunostimulatory property was analysed. When inoculated in BALB/c mice, this peptide induced high levels of SmB2LJ-specific IgG1 and IgG2a subtypes, as detected by enzyme linked immunosorbent assay. In addition, dot blots were found to be positive for immune sera against potato apyrase and SmB2LJ. These results suggest that the conserved domain r175-194 from the S. mansoni SmATPDase 2 is antigenic. Western blots were performed and the anti-SmB2LJ antibody recognised in adult worm (soluble worm antigen preparation) or soluble egg antigen antigenic preparations two bands of approximately 63 and 55 kDa, molecular masses similar to those predicted for adult worm SmATPDase 2. This finding strongly suggests the expression of this same isoform in S. mansoni eggs. To assess localisation of SmATPDase 2, confocal fluorescence microscopy was performed using cryostat sections of infected mouse liver and polyclonal antiserum against SmB2LJ. Positive reactions were identified on the external surface from the miracidium in von Lichtenberg's envelope and, in the outer side of the egg-shell, showing that this soluble isoform is secreted from the S. mansoni eggs.en
dc.description.affiliationUniversidade Federal de Juiz de Fora Instituto de Ciências Biológicas Departamento de Bioquímica
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Biofísica
dc.description.affiliationInstituto de Pesquisas René Rachou-Fiocruz
dc.description.affiliationInstituto Oswaldo Cruz-Fiocruz Laboratório de Patologia
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Biofísica
dc.description.sourceSciELO
dc.format.extent808-813
dc.identifierhttp://dx.doi.org/10.1590/S0074-02762011000700005
dc.identifier.citationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 7, p. 808-813, 2011.
dc.identifier.doi10.1590/S0074-02762011000700005
dc.identifier.fileS0074-02762011000700005.pdf
dc.identifier.issn0074-0276
dc.identifier.scieloS0074-02762011000700005
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/6685
dc.identifier.wosWOS:000297789800005
dc.language.isoeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz
dc.rightsAcesso aberto
dc.subjectSchistosoma mansonien
dc.subjectNTPDaseen
dc.subjectpotato apyraseen
dc.subjectpeptideen
dc.titleImmunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni eggen
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