Mesenchymal Stem Cells (MSC) Prevented the Progression of Renovascular Hypertension, Improved Renal Function and Architecture

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dc.contributor.authorOliveira-Sales, Elizabeth Barbosa de [UNIFESP]
dc.contributor.authorMaquigussa, Edgar [UNIFESP]
dc.contributor.authorSemedo, Patricia [UNIFESP]
dc.contributor.authorPereira, Luciana G. [UNIFESP]
dc.contributor.authorFerreira, Vanessa M. [UNIFESP]
dc.contributor.authorCâmara, Niels Olsen Saraiva [UNIFESP]
dc.contributor.authorBergamaschi, Cassia Toledo [UNIFESP]
dc.contributor.authorCampos, Ruy Ribeiro [UNIFESP]
dc.contributor.authorBoim, Mirian Aparecida [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:34:42Z
dc.date.available2016-01-24T14:34:42Z
dc.date.issued2013-11-04
dc.description.abstractRenovascular hypertension induced by 2 Kidney-1 Clip (2K-1C) is a renin-angiotensin-system (RAS)-dependent model, leading to renal vascular rarefaction and renal failure. RAS inhibitors are not able to reduce arterial pressure (AP) and/or preserve the renal function, and thus, alternative therapies are needed. Three weeks after left renal artery occlusion, fluorescently tagged mesenchymal stem cells (MSC) (2x10(5) cells/animal) were injected weekly into the tail vein in 2K-1C hypertensive rats. Flow cytometry showed labeled MSC in the cortex and medulla of the clipped kidney. MSC prevented a further increase in the AP, significantly reduced proteinuria and decreased sympathetic hyperactivity in 2K-1C rats. Renal function parameters were unchanged, except for an increase in urinary volume observed in 2K-1C rats, which was not corrected by MSC. the treatment improved the morphology and decreased the fibrotic areas in the clipped kidney and also significantly reduced renal vascular rarefaction typical of 2K-1C model. Expression levels of IL-1 beta, TNF-alpha angiotensinogen, ACE, and Ang II receptor AT(1) were elevated, whereas AT(2) levels were decreased in the medulla of the clipped kidney. MSC normalized these expression levels. in conclusion, MSC therapy in the 2K-1C model (i) prevented the progressive increase of AP, (ii) improved renal morphology and microvascular rarefaction, (iii) reduced fibrosis, proteinuria and inflammatory cytokines, (iv) suppressed the intrarenal RAS, iv) decreased sympathetic hyperactivity in anesthetized animals and v) MSC were detected at the CNS suggesting that the cells crossed the blood-brain barrier. This therapy may be a promising strategy to treat renovascular hypertension and its renal consequences in the near future.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Med, Div Renal, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Div Cardiovasc, Dept Physiol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Immunol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Med, Div Renal, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Cardiovasc, Dept Physiol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent10
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0078464
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 8, n. 11, 10 p., 2013.
dc.identifier.doi10.1371/journal.pone.0078464
dc.identifier.fileWOS000326503400064.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36971
dc.identifier.wosWOS:000326503400064
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleMesenchymal Stem Cells (MSC) Prevented the Progression of Renovascular Hypertension, Improved Renal Function and Architectureen
dc.typeinfo:eu-repo/semantics/article
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