Use of transgenic (knockout) mice reveals a site distinct from the alpha(2A)-adrenoceptors for agmatine in the vas deferens

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dc.contributor.authorSantos, Wilson da Costa
dc.contributor.authorGarcez-do-Carmo, Lucia [UNIFESP]
dc.contributor.authorSilva, Eliane Cristina da [UNIFESP]
dc.contributor.authorPascual, Ricardo de
dc.contributor.authorJurkiewicz, Neide Hyppolito [UNIFESP]
dc.contributor.authorJurkiewicz, Aron [UNIFESP]
dc.contributor.authorGandia, Luis
dc.contributor.institutionUniversidade Federal Fluminense (UFF)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFac Med
dc.date.accessioned2018-06-18T11:27:00Z
dc.date.available2018-06-18T11:27:00Z
dc.date.issued2009-03-01
dc.description.abstractThe inhibitory effect of agmatine on electrically induced contractions was studied in vas deferens of Adra 2a transgenic mice lacking alpha(2A)-adrenoceptors. Agmatine and clonidine caused a concentration-dependent inhibition of twitches. However, while agmatine showed a similar pIC(50) value in control and transgenic mice, the pIC(50) value for clonidine was about 30-fold lower in knockout mice. In both strains, yohimbine shifted the curve for clonidine, but not for agmatine, even when a 100-fold higher concentration of yohimbine was employed. Our results indicate that inhibition by agmatine in mouse vas deferens is not simply due to interactions with alpha(2)-adrenoceptors in our experimental conditions.en
dc.description.affiliationUniv Fed Fluminense, Fac Farm, Dept Farm & Adm Farmaceut, BR-24241000 Niteroi, RJ, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Farmacol, BR-04021023 Sao Paulo, Brazil
dc.description.affiliationFac Med, Dept Farmacol & Terapeut, Inst Teofilo Hernando, Madrid 28029, Spain
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Farmacol, BR-04021023 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacion Teofilo Hernando (Spain)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent325-329
dc.identifierhttp://dx.doi.org/10.1016/S1734-1140(09)70039-8
dc.identifier.citationPharmacological Reports. Krakow: Polish Acad Sciences Inst Pharmacology, v. 61, n. 2, p. 325-329, 2009.
dc.identifier.doi10.1016/S1734-1140(09)70039-8
dc.identifier.issn1734-1140
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/45087
dc.identifier.wosWOS:000266134200016
dc.language.isoeng
dc.publisherPolish Acad Sciences Inst Pharmacology
dc.relation.ispartofPharmacological Reports
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectvas deferensen
dc.subjecttransgenic (knockout) miceen
dc.subjectagmatineen
dc.subjectalpha(2A)-adrenoceptorsen
dc.subjectneurotransmissionen
dc.titleUse of transgenic (knockout) mice reveals a site distinct from the alpha(2A)-adrenoceptors for agmatine in the vas deferensen
dc.typeinfo:eu-repo/semantics/article
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