Use of transgenic (knockout) mice reveals a site distinct from the alpha(2A)-adrenoceptors for agmatine in the vas deferens

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2009-03-01
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Santos, Wilson da Costa
Garcez-do-Carmo, Lucia [UNIFESP]
Silva, Eliane Cristina da [UNIFESP]
Pascual, Ricardo de
Jurkiewicz, Neide Hyppolito [UNIFESP]
Jurkiewicz, Aron [UNIFESP]
Gandia, Luis
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The inhibitory effect of agmatine on electrically induced contractions was studied in vas deferens of Adra 2a transgenic mice lacking alpha(2A)-adrenoceptors. Agmatine and clonidine caused a concentration-dependent inhibition of twitches. However, while agmatine showed a similar pIC(50) value in control and transgenic mice, the pIC(50) value for clonidine was about 30-fold lower in knockout mice. In both strains, yohimbine shifted the curve for clonidine, but not for agmatine, even when a 100-fold higher concentration of yohimbine was employed. Our results indicate that inhibition by agmatine in mouse vas deferens is not simply due to interactions with alpha(2)-adrenoceptors in our experimental conditions.
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Pharmacological Reports. Krakow: Polish Acad Sciences Inst Pharmacology, v. 61, n. 2, p. 325-329, 2009.
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