Development of experimental in vitro burn model

dc.contributor.authorFernandes, Ana Carolina Morais
dc.contributor.authorFrança, Jerônimo Pereira de [UNIFESP]
dc.contributor.authorGaiba, Silvana
dc.contributor.authorAloise, Antonio Carlos [UNIFESP]
dc.contributor.authorOliveira, Andrea Fernandes de
dc.contributor.authorMoraes, Andrea Aparecida Fátima Souza [UNIFESP]
dc.contributor.authorFrança, Lucimar Pereira de
dc.contributor.authorFerreira, Lydia Masako [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Santa Cruz Department of Biological Sciences
dc.date.accessioned2015-06-14T13:46:49Z
dc.date.available2015-06-14T13:46:49Z
dc.date.issued2014-01-01
dc.description.abstractPURPOSE:To propose an experimental burn model in NIH-3T3 cell line.METHODS: Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence.RESULTS: The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group.CONCLUSION: The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniversidade Estadual de Santa Cruz Department of Biological Sciences
dc.description.affiliationUNIFESP
dc.description.affiliationUnifespUNIFESP
dc.description.sourceSciELO
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent15-20
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502014001400004
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 15-20, 2014.
dc.identifier.doi10.1590/S0102-86502014001400004
dc.identifier.fileS0102-86502014001400005.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502014001400005
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8144
dc.identifier.wosWOS:000342065300005
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsAcesso aberto
dc.subjectBurnen
dc.subjectfibroblasten
dc.subjectcell line NIH-3T3en
dc.subjectmicrowaveen
dc.subjectMTTen
dc.subjectConfocal microscopyen
dc.titleDevelopment of experimental in vitro burn modelen
dc.typeArtigo
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